Anti-SPTLC1 antibody (ab264321)
Key features and details
- Rabbit polyclonal to SPTLC1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SPTLC1 antibody
See all SPTLC1 primary antibodies -
Description
Rabbit polyclonal to SPTLC1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human SPTLC1 aa 423-473. The exact sequence is proprietary. NP_006406.1
Database link: O15269 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SPTLC1 antibody (ab264321)All lanes : Anti-SPTLC1 antibody (ab264321) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HEK-293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 53 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-SPTLC1 antibody (ab264321)SPTLC1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264321 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264321 at 1 µg/ml.
Lane 1: ab264321 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 30 secs.
