Anti-SEPT7 antibody (ab244434)
Key features and details
- Rabbit polyclonal to SEPT7
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SEPT7 antibody
See all SEPT7 primary antibodies -
Description
Rabbit polyclonal to SEPT7 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Orangutan
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Immunogen
Recombinant fragment corresponding to Human SEPT7 aa 150-300.
Database link: Q16181 -
Positive control
- WB: RT4, U-2 OS, NIH/3T3, NBT II and U-251 MG cell lysates; human liber and tonsil tissue lysates. IHC-P: Human fallopian tube, cerebral cortex, testis and lymph node tissue. ICC/IF: A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SEPT7 antibody (ab244434)All lanes : Anti-SEPT7 antibody (ab244434) at 0.4 µg/ml
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line9 cell lysate
Lane 2 : NBT II (rat cell line) cell lysate
Predicted band size: 51 kDa
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Western blot - Anti-SEPT7 antibody (ab244434)Anti-SEPT7 antibody (ab244434) at 0.4 µg/ml + U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate
Predicted band size: 51 kDa
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Western blot - Anti-SEPT7 antibody (ab244434)All lanes : Anti-SEPT7 antibody (ab244434) at 0.4 µg/ml
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 51 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT7 antibody (ab244434)Paraffin-embedded human lymph node tissue stained for SEPT7 using ab244434 at 1/500 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT7 antibody (ab244434)Paraffin-embedded human testis tissue stained for SEPT7 using ab244434 at 1/500 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT7 antibody (ab244434)Paraffin-embedded human skeletal muscle tissue stained for SEPT7 using ab244434 at 1/500 dilution in immunohistochemical analysis. No positivity in myocytes, as expected.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT7 antibody (ab244434)Paraffin-embedded human cerebral cortex tissue stained for SEPT7 using ab244434 at 1/500 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEPT7 antibody (ab244434)Paraffin-embedded human fallopian tube tissue stained for SEPT7 using ab244434 at 1/500 dilution in immunohistochemical analysis. -
Immunocytochemistry/ Immunofluorescence - Anti-SEPT7 antibody (ab244434)PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for SEPT7 (green) using ab244434 at 4 μg/ml in ICC/IF.
