LysoLive™ Lysosomal Beta-Glucosidase Assay Kit (ab253373)
Key features and details
- Detection method: Fluorescent
Overview
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Product name
LysoLive™ Lysosomal Beta-Glucosidase Assay Kit
See all beta-Glucosidase kits -
Detection method
Fluorescent -
Product overview
This kit utilizes the sensitive substrate GlucGreen™ to monitor beta-glucosidase activity in the lysosomes of live cells.
Lysosomal beta-glucosidase enzyme is currently indicated to be involved in diseases such as Gaucher and Parkinson’s diseases. LysoLive™ Lysosomal beta-Glucosidase Assay Kit utilizes a substrate which only becomes fluorescent upon cleavage by the enzyme meaning lysosomes with low beta-Glucosidase (GBA) activity will exhibit reduced staining in a manner proportional to the enzyme activity levels in the cell.
This kit is sold by Marker Gene under product code M2775.
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Notes
Important Licensing Information: This product may be covered by one or more United States or International Patents and is subject to our Limited Use Label Licenses. By use of this product, you accept the terms and conditions of all applicable Limited Use Label Licenses.
Properties
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Storage instructions
Please refer to protocols. -
Components 1 units LysoLive™ GlucGreen 5 x 10µl Opti-Klear™ Live Cell Imaging Buffer 1X 2 x 30ml
Images
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Labeling cells with LysoLive™ Lysosomal ß-Glucosidase Assay Kit in healthy cells (left) and those from a Gaucher Disease patient (right).
5µM LysoLive™ GlucGreen™, 16 hour incubation.
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Hela and healthy immortalized B-Lymphoblasts were labeled with LysoLive™ Lysosomal ß-Glucosidase Assay Kit at 5µM for 16 hours.Media was then replaced with Opti-Klear Live Cell Imaging buffer containing Hoechst 33342 at 10 µg/ml.
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HeLa cells were cultured with CBE for 7-10 days.LysoLive GlucGreen was added at a final concentration of 5µM in complete media and incubated for 16 hours. Next day, cells were washed once with 1X PBS and bathed cells in Opti-Klear Imaging Buffer containing Hoechst 33342 healthy leukocytes (bottom) were treated with CBE for 16 hours in complete media containing 5uM final concentration LysoLive GlucGreen. Next day, cells were washed once with 1x PBS and bathed cells in Opti-Klear Imaging Buffer containing Hoechst 33342.
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HeLa cells (top) and healthy leukocytes (bottom) treated with chloroquine for 16 hours in complete media containing 5µM final concentration LysoLive GlucGreen.Next day, cells were washed once with 1x PBS and bathed cells in Opti-Klear Imaging Buffer containing Hoechst 33342.