Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UQCRB antibody [EPR15591] (ab190360)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15591] to UQCRB
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-UQCRB antibody [EPR15591]
    See all UQCRB primary antibodies

  • Description

    Rabbit monoclonal [EPR15591] to UQCRB

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, Human fetal heart, 293, mouse heart, rat brain and NIH-3T3 lysates, Human adenocarcinoma of colon tissue, HeLa cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-UQCRB antibody [EPR15591] (ab190360)
    Western blot - Anti-UQCRB antibody [EPR15591] (ab190360)
    All lanes : Anti-UQCRB antibody [EPR15591] (ab190360) at 1/20000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Human fetal heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 14 kDa
    Observed band size: 14 kDa

  • Western blot - Anti-UQCRB antibody [EPR15591] (ab190360)
    Western blot - Anti-UQCRB antibody [EPR15591] (ab190360)
    Anti-UQCRB antibody [EPR15591] (ab190360) at 1/5000 dilution + 293 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 14 kDa
    Observed band size: 14 kDa

  • Western blot - Anti-UQCRB antibody [EPR15591] (ab190360)
    Western blot - Anti-UQCRB antibody [EPR15591] (ab190360)
    All lanes : Anti-UQCRB antibody [EPR15591] (ab190360) at 1/1000 dilution

    Lane 1 : Mouse heart
    Lane 2 : Rat brain
    Lane 3 : NIH-3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 14 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRB antibody [EPR15591] (ab190360)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRB antibody [EPR15591] (ab190360)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human adenocarcinoma of the colon labeling UQCRB with ab190360 at 1/100 and HRP Polymer for Rabbit IgG.  Counterstained with hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-UQCRB antibody [EPR15591] (ab190360)
    Immunocytochemistry/ Immunofluorescence - Anti-UQCRB antibody [EPR15591] (ab190360)

    Immunocytochemical analysis of HeLa cells fixed in 4% paraformaldehyde labeling UQCRB with ab190360 at 1/250 dilution and Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution.  Counterstained with DAPI. Negative controls also shown.

  • Anti-UQCRB antibody [EPR15591] (ab190360)
    Anti-UQCRB antibody [EPR15591] (ab190360)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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