Antibodies, Proteins, Kits and Reagents for Life Science

Anti-ACAA1/Beta-ketothiolase antibody [CL2650] (ab210961)

Key features and details

Mouse monoclonal [CL2650] to ACAA1/Beta-ketothiolase
Suitable for: IHC-P, WB, ICC/IF
Reacts with: Human
Isotype: IgG1

Product name

Anti-ACAA1/Beta-ketothiolase antibody [CL2650]
See all ACAA1/Beta-ketothiolase primary antibodies
Description

Mouse monoclonal [CL2650] to ACAA1/Beta-ketothiolase
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment (proprietary-tag) corresponding to Human ACAA1/Beta-ketothiolase aa 50-200.
Database link: P09110

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Epitope

Binds to an epitope located within the peptide sequence GAIMARIAQFLSDIP as determined by overlapping synthetic peptides.
Positive control
- IHC-P: Human colon, liver, kidney and prostate tissues. WB: U-251 Cells ICC/IF: CACO-2
General notes

This product was previously labelled as ACAA1

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

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Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

CL2650
Isotype

IgG1
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-ACAA1/Beta-ketothiolase antibody [CL2650] (ab210961)

PFA-fixed, Triton X-100 permeabilized CACO-2 cells stained for ACAA1 (Green) using ab210961 at 1 µg/ml. Microtubules are stained red, nuclei are stained blue.
Western blot - Anti-ACAA1/Beta-ketothiolase antibody [CL2650] (ab210961)

Anti-ACAA1/Beta-ketothiolase antibody [CL2650] (ab210961) at 1/500 dilution + U251 cells

Predicted band size: 44 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1/Beta-ketothiolase antibody [CL2650] (ab210961)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human prostate tissue labeling ACAA1/Beta-ketothiolase with ab210961 at 1/200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1/Beta-ketothiolase antibody [CL2650] (ab210961)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human kidney tissue labeling ACAA1/Beta-ketothiolase with ab210961 at 1/200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1/Beta-ketothiolase antibody [CL2650] (ab210961)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human liver tissue labeling ACAA1/Beta-ketothiolase with ab210961 at 1/200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACAA1/Beta-ketothiolase antibody [CL2650] (ab210961)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human colon tissue labeling ACAA1/Beta-ketothiolase with ab210961 at 1/200 dilution.

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