Antibodies, Proteins, Kits and Reagents for Life Science

334 369 ₸ Product size

100 µl 334 369 ₸

Order now and get it on Thursday February 25, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
HRP Rabbit monoclonal [E63] to Bax
Suitable for: WB
Knockout validated
Reacts with: Human
Conjugation: HRP

Product name

HRP Anti-Bax antibody [E63]
See all Bax primary antibodies
Description

HRP Rabbit monoclonal [E63] to Bax
Host species

Rabbit
Conjugation

HRP
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human Bax aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q07812
Positive control
- WB: HeLa and wildtype HAP1 whole cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage buffer

pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

E63
Isotype

IgG
Research areas

Western blot - HRP Anti-Bax antibody [E63] (ab205822)

All lanes : HRP Anti-Bax antibody [E63] (ab205822) at 1/5000 dilution

Lane 1 : HeLa whole cell lysate (ab150035) at 10 µg
Lane 2 : Wild-type HAP1 cell lysate at 20 µg
Lane 3 : Bax knockout HAP1 cell lysate at 20 µg

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 21 kDa
Observed band size: 19 kDa
why is the actual band size different from the predicted?

Exposure time: 8 minutes

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205822 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
HRP Anti-Bax antibody [E63] (ab205822)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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