Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Bax antibody (ab104156)

Key features and details

  • Rabbit polyclonal to Bax
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Bax antibody
    See all Bax primary antibodies

  • Description

    Rabbit polyclonal to Bax

  • Host species

    Rabbit

  • Specificity

    Expression levels of BAX protein vary with sample type. Induction may be required if endogenous expression is low.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Bax aa 1-100 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q07812

  • Positive control

    • This antibody gave a positive signal in Human lymph node tissue lysate.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab104156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Cow
Cat
Dog
Pig
Macaque monkey
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).

Target

  • Function

    Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

  • Tissue specificity

    Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.

  • Sequence similarities

    Belongs to the Bcl-2 family.

  • Domain

    Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.

  • Cellular localization

    Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Apoptosis regulator BAX antibody
      • BAX antibody
      • Bax-protein antibody
      • BAX_HUMAN antibody
      • BAXA antibody
      • Baxdelta2G9 antibody
      • Baxdelta2G9omega antibody
      • Baxdelta2omega antibody
      • Bcl-2-like protein 4 antibody
      • BCL2 associated X protein antibody
      • BCL2 associated X protein omega antibody
      • BCL2 associated X protein transcript variant delta2 antibody
      • Bcl2-L-4 antibody
      • BCL2L4 antibody
      • membrane isoform alpha antibody
      see all

    Images

    • Western blot - Anti-Bax antibody (ab104156)
      Western blot - Anti-Bax antibody (ab104156)

      Lanes 1, 3 and 5: Wild-type HAP1 cell lysate (20 µg)
      Lanes 2, 4 and 6: Bax knockout HAP1 cell lysate (20 µg)
      Lanes 1 and 2: Green signal from target – ab104156 observed at 20 kDa
      Lanes 3 and 4: Red signal from loading control – ab8226 observed at 42 kDa
      Lanes 5 and 6: Merged (red and green) signal

      ab104156 was shown to react with Bax when Bax knockout samples were used, along with additional cross-reactive bands. Wild-type and Bax knockout samples were subjected to SDS-PAGE. ab104156 and ab8226 (loading control to beta actin) were diluted 1 µg/mL and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • Western blot - Anti-Bax antibody (ab104156)
      Western blot - Anti-Bax antibody (ab104156)
      Anti-Bax antibody (ab104156) at 1 µg/ml + Human lymph node tissue lysate - total protein (ab29871) at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 21 kDa
      Observed band size: 21 kDa


      Exposure time: 4 minutes
    • Western blot - Anti-Bax antibody (ab104156)
      Western blot - Anti-Bax antibody (ab104156)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Bax knockout HAP1 cell lysate (20 µg)

      Lanes 1 - 2: Merged signal (red and green). Green - ab104156 observed at 20 kDa. Red - loading control, ab8245, observed at 37 kDa or ab18058, observed at 130 kDa.

      This western blot image is a comparison between ab104156 and a competitor's top cited rabbit polyclonal antibody.

    References (3)

    Publishing research using ab104156? Please let us know so that we can cite the reference in this datasheet.

    ab104156 has been referenced in 3 publications.

    • Wang X  et al. Silence of lncRNA ANRIL represses cell growth and promotes apoptosis in retinoblastoma cells through regulating miR-99a and c-Myc. Artif Cells Nanomed Biotechnol 47:2265-2273 (2019). PubMed: 31184221
    • Zhang Z  et al. Resveratrol attenuates mechanical compression-induced nucleus pulposus cell apoptosis through regulating the ERK1/2 signaling pathway in a disc organ culture. Biosci Rep 38:N/A (2018). PubMed: 29439139
    • Xu H  et al. Downregulation of microRNA-429 protects cardiomyocytes against hypoxia-induced apoptosis by increasing Notch1 expression. Int J Mol Med 37:1677-85 (2016). PubMed: 27082497

    Images

    • Western blot - Anti-Bax antibody (ab104156)
      Western blot - Anti-Bax antibody (ab104156)

      Lanes 1, 3 and 5: Wild-type HAP1 cell lysate (20 µg)
      Lanes 2, 4 and 6: Bax knockout HAP1 cell lysate (20 µg)
      Lanes 1 and 2: Green signal from target – ab104156 observed at 20 kDa
      Lanes 3 and 4: Red signal from loading control – ab8226 observed at 42 kDa
      Lanes 5 and 6: Merged (red and green) signal

      ab104156 was shown to react with Bax when Bax knockout samples were used, along with additional cross-reactive bands. Wild-type and Bax knockout samples were subjected to SDS-PAGE. ab104156 and ab8226 (loading control to beta actin) were diluted 1 µg/mL and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • Western blot - Anti-Bax antibody (ab104156)
      Western blot - Anti-Bax antibody (ab104156)
      Anti-Bax antibody (ab104156) at 1 µg/ml + Human lymph node tissue lysate - total protein (ab29871) at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 21 kDa
      Observed band size: 21 kDa


      Exposure time: 4 minutes
    • Western blot - Anti-Bax antibody (ab104156)
      Western blot - Anti-Bax antibody (ab104156)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Bax knockout HAP1 cell lysate (20 µg)

      Lanes 1 - 2: Merged signal (red and green). Green - ab104156 observed at 20 kDa. Red - loading control, ab8245, observed at 37 kDa or ab18058, observed at 130 kDa.

      This western blot image is a comparison between ab104156 and a competitor's top cited rabbit polyclonal antibody.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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