Anti-Angiopoietin 2/ANG2 antibody [117] - BSA and Azide free (ab276042)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [117] to Angiopoietin 2/ANG2 - BSA and Azide free
- Suitable for: WB, ICC, IP
- Reacts with: Human
Overview
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Product name
Anti-Angiopoietin 2/ANG2 antibody [117] - BSA and Azide free
See all Angiopoietin 2/ANG2 primary antibodies -
Description
Rabbit monoclonal [117] to Angiopoietin 2/ANG2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein (His-tag) corresponding to Human Angiopoietin 2/ANG2 aa 1-496 (C terminal). NP_001138.1
Database link: O15123 -
Positive control
- ICC: A549 cells. WB: HepG2 and A549 whole cell lysates IP: HepG2, A549 and HEK-293T whole cell lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
117 -
Isotype
IgG -
Research areas
Images
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Immunofluorescence staining of Angiopoietin 2/ANG2 in A549 (Angiopoietin 2/ANG2) cells. Cells were fixed with 4% PFA, permeabilized with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with ab276042 at 1/300 at 4 °C overnight. Then cells were stained with the AlexaFluor® 549-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).
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All lanes : Anti-Angiopoietin 2/ANG2 antibody [117] - BSA and Azide free (ab276042) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : A549 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution
Predicted band size: 56 kDaPerformed under reducing conditions.
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Angiopoietin 2/ANG2 was immunoprecipitated from the following whole cell lysates:
Lane A:0.5 mg HepG2 (Angiopoietin 2/ANG2) whole Cell Lysate
Lane B:0.5 mg A549 (Angiopoietin 2/ANG2) whole Cell Lysate
Lane C:0.5 mg HEK-293T (Angiopoietin 2/ANG2) whole Cell Lysate0.5 µL ab276042 and 15 µl of 50 % Protein G agarose.
Primary antibody: ab276042, at 1/500 dilution
Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1/5000 dilution -