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Anti-Angiopoietin 2/ANG2 antibody [117] - BSA and Azide free (ab276042)

Price and availability

177 571 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-Angiopoietin 2/ANG2 antibody [117] - BSA and Azide free (ab276042)
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  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [117] to Angiopoietin 2/ANG2 - BSA and Azide free
  • Suitable for: WB, ICC, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-Angiopoietin 2/ANG2 antibody [117] - BSA and Azide free
    See all Angiopoietin 2/ANG2 primary antibodies
  • Description

    Rabbit monoclonal [117] to Angiopoietin 2/ANG2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein (His-tag) corresponding to Human Angiopoietin 2/ANG2 aa 1-496 (C terminal). NP_001138.1
    Database link: O15123

  • Positive control

    • ICC: A549 cells. WB: HepG2 and A549 whole cell lysates IP: HepG2, A549 and HEK-293T whole cell lysates.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Constituent: 100% PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    117
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • Angiopoietin

Images

  • Immunocytochemistry - Anti-Angiopoietin 2/ANG2 antibody [117] (ab276042)
    Immunocytochemistry - Anti-Angiopoietin 2/ANG2 antibody [117] (ab276042)

    Immunofluorescence staining of Angiopoietin 2/ANG2 in A549 (Angiopoietin 2/ANG2) cells. Cells were fixed with 4% PFA, permeabilized with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with ab276042 at 1/300 at 4 °C overnight. Then cells were stained with the AlexaFluor® 549-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).

  • Western blot - Anti-Angiopoietin 2/ANG2 antibody [117] (ab276042)
    Western blot - Anti-Angiopoietin 2/ANG2 antibody [117] (ab276042)
    All lanes : Anti-Angiopoietin 2/ANG2 antibody [117] - BSA and Azide free (ab276042) at 1/500 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : A549 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

    Predicted band size: 56 kDa



    Performed under reducing conditions.

  • Immunoprecipitation - Anti-Angiopoietin 2/ANG2 antibody [117] (ab276042)
    Immunoprecipitation - Anti-Angiopoietin 2/ANG2 antibody [117] (ab276042)

    Angiopoietin 2/ANG2 was immunoprecipitated from the following whole cell lysates:

    Lane A:0.5 mg HepG2 (Angiopoietin 2/ANG2) whole Cell Lysate
    Lane B:0.5 mg A549 (Angiopoietin 2/ANG2) whole Cell Lysate
    Lane C:0.5 mg HEK-293T (Angiopoietin 2/ANG2) whole Cell Lysate

    0.5 µL ab276042 and 15 µl of 50 % Protein G agarose.
    Primary antibody: ab276042, at 1/500 dilution
    Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1/5000 dilution


  • Anti-Angiopoietin 2/ANG2 antibody [117] - BSA and Azide free (ab276042)
    Anti-Angiopoietin 2/ANG2 antibody [117] - BSA and Azide free (ab276042)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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