Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-COX5B antibody [EPR14439(B)] - BSA and Azide free (ab238984)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14439(B)] to COX5B - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-COX5B antibody [EPR14439(B)] - BSA and Azide free
    See all COX5B primary antibodies

  • Description

    Rabbit monoclonal [EPR14439(B)] to COX5B - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human kidney tissue.

  • General notes

    ab238984 is the carrier-free version of ab180136.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-COX5B antibody [EPR14439(B)] - BSA and Azide free (ab238984)
    Flow Cytometry - Anti-COX5B antibody [EPR14439(B)] - BSA and Azide free (ab238984)

    Flow cytometry analysis of HeLa cells labeling COX5B using ab180136 at 1/10 dilution. A Goat anti rabbit IgG (FITC) at 1/75 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180136).

  • Immunocytochemistry/ Immunofluorescence - Anti-COX5B antibody [EPR14439(B)] - BSA and Azide free (ab238984)
    Immunocytochemistry/ Immunofluorescence - Anti-COX5B antibody [EPR14439(B)] - BSA and Azide free (ab238984)

    Immunofluorescence analysis of HepG2 cells labeling COX5B using ab180136 at 1/1000 dilution. A Goat anti rabbit IgG (Dylight 555) at 1/250 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain: DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180136).

  • Immunoprecipitation - Anti-COX5B antibody [EPR14439(B)] - BSA and Azide free (ab238984)
    Immunoprecipitation - Anti-COX5B antibody [EPR14439(B)] - BSA and Azide free (ab238984)

    Immunoprecipitation analysis of Human fetal heart lysate labeling COX5B using ab180136 at 1/70 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180136).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX5B antibody [EPR14439(B)] - BSA and Azide free (ab238984)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX5B antibody [EPR14439(B)] - BSA and Azide free (ab238984)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling COX5B using ab180136 at 1/500 dilution. A Goat anti rabbit IgG (HRP) prediluted was used as secondary. Counterstain: Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180136).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-COX5B antibody [EPR14439(B)] - BSA and Azide free (ab238984)
    Anti-COX5B antibody [EPR14439(B)] - BSA and Azide free (ab238984)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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