Anti-COX5B antibody (ab264401)
Key features and details
- Rabbit polyclonal to COX5B
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-COX5B antibody
See all COX5B primary antibodies -
Description
Rabbit polyclonal to COX5B -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman
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Immunogen
Synthetic peptide within Human COX5B aa 25-75. The exact sequence is proprietary.
Database link: P10606 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-COX5B antibody (ab264401)All lanes : Anti-COX5B antibody (ab264401) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 14 kDa
Exposure time: 3 minutesLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-COX5B antibody (ab264401)COX5B was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264401 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab264401 at 0.4 µg/ml.
Lane 1: ab264401 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with an exposure time of 30 seconds.
Lysates prepared using NETN lysis buffer.
