Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-COX5B antibody [EPR14439(B)] (ab180136)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14439(B)] to COX5B
  • Suitable for: Flow Cyt, IHC-P, WB, ICC/IF, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-COX5B antibody [EPR14439(B)]
    See all COX5B primary antibodies

  • Description

    Rabbit monoclonal [EPR14439(B)] to COX5B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human COX5B aa 1-100. The exact sequence is proprietary.
    Database link: P10606

  • Positive control

    • Human fetal heart lysate; HeLa and HepG2 cells; Human kidney tissue; U87-MG, HepG2 and HeLa cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-COX5B antibody [EPR14439(B)] (ab180136)
    Western blot - Anti-COX5B antibody [EPR14439(B)] (ab180136)
    All lanes : Anti-COX5B antibody [EPR14439(B)] (ab180136) at 1/50000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 14 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-COX5B antibody [EPR14439(B)] (ab180136)
    Immunocytochemistry/ Immunofluorescence - Anti-COX5B antibody [EPR14439(B)] (ab180136)

    Immunofluorescence analysis of HepG2 cells labeling COX5B using ab180136 at 1/1000 dilution. A Goat anti rabbit IgG (Dylight 555) at 1/250 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain: DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX5B antibody [EPR14439(B)] (ab180136)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX5B antibody [EPR14439(B)] (ab180136)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling COX5B using ab180136 at 1/500 dilution. A Goat anti rabbit IgG (HRP) prediluted was used as secondary. Counterstain: Hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-COX5B antibody [EPR14439(B)] (ab180136)
    Immunoprecipitation - Anti-COX5B antibody [EPR14439(B)] (ab180136)

    Immunoprecipitation analysis of Human fetal heart lysate labeling COX5B using ab180136 at 1/70 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.

  • Western blot - Anti-COX5B antibody [EPR14439(B)] (ab180136)
    Western blot - Anti-COX5B antibody [EPR14439(B)] (ab180136)
    Anti-COX5B antibody [EPR14439(B)] (ab180136) at 1/10000 dilution + U87-MG cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 14 kDa

  • Flow Cytometry - Anti-COX5B antibody [EPR14439(B)] (ab180136)
    Flow Cytometry - Anti-COX5B antibody [EPR14439(B)] (ab180136)

    Flow cytometry analysis of HeLa cells labeling COX5B using ab180136 at 1/10 dilution. A Goat anti rabbit IgG (FITC) at 1/75 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit IgG.

  • Anti-COX5B antibody [EPR14439(B)] (ab180136)
    Anti-COX5B antibody [EPR14439(B)] (ab180136)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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