Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 02, 2021

Anti-COX5B antibody [EPR14439(B)] (ab180136)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14439(B)] to COX5B
Suitable for: Flow Cyt, IHC-P, WB, ICC/IF, IP
Reacts with: Human

Product name

Anti-COX5B antibody [EPR14439(B)]
See all COX5B primary antibodies
Description

Rabbit monoclonal [EPR14439(B)] to COX5B
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human COX5B aa 1-100. The exact sequence is proprietary.
Database link: P10606
Positive control
- Human fetal heart lysate; HeLa and HepG2 cells; Human kidney tissue; U87-MG, HepG2 and HeLa cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR14439(B)
Isotype

IgG
Research areas

Western blot - Anti-COX5B antibody [EPR14439(B)] (ab180136)

All lanes : Anti-COX5B antibody [EPR14439(B)] (ab180136) at 1/50000 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 14 kDa
Immunocytochemistry/ Immunofluorescence - Anti-COX5B antibody [EPR14439(B)] (ab180136)

Immunofluorescence analysis of HepG2 cells labeling COX5B using ab180136 at 1/1000 dilution. A Goat anti rabbit IgG (Dylight 555) at 1/250 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain: DAPI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX5B antibody [EPR14439(B)] (ab180136)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling COX5B using ab180136 at 1/500 dilution. A Goat anti rabbit IgG (HRP) prediluted was used as secondary. Counterstain: Hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-COX5B antibody [EPR14439(B)] (ab180136)

Immunoprecipitation analysis of Human fetal heart lysate labeling COX5B using ab180136 at 1/70 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.
Western blot - Anti-COX5B antibody [EPR14439(B)] (ab180136)

Anti-COX5B antibody [EPR14439(B)] (ab180136) at 1/10000 dilution + U87-MG cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 14 kDa
Flow Cytometry - Anti-COX5B antibody [EPR14439(B)] (ab180136)

Flow cytometry analysis of HeLa cells labeling COX5B using ab180136 at 1/10 dilution. A Goat anti rabbit IgG (FITC) at 1/75 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit IgG.
Anti-COX5B antibody [EPR14439(B)] (ab180136)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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