Anti-CD130 (gp130) antibody (ab202850)
Key features and details
- Rabbit polyclonal to CD130 (gp130)
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CD130 (gp130) antibody
See all CD130 (gp130) primary antibodies -
Description
Rabbit polyclonal to CD130 (gp130) -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Mouse CD130 (gp130) aa 650-750 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q00560 -
Positive control
- IHC-P: Human endometrial cancer tissues. WB: Mouse lung tissue lysate. Flow Cyt: Raji cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.01% BSA
Aqueous buffered solution -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CD130 (gp130) antibody (ab202850)Anti-CD130 (gp130) antibody (ab202850) at 1/300 dilution + Mouse lung tissue lysate
Secondary
Conjugated secondary antibody at 1/5000 dilution
Predicted band size: 102 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD130 (gp130) antibody (ab202850)Immunohistochemical analysis of formalin-fixed paraffin-embedded human endometrial cancer tissue labeling CD130 (gp130) with ab202850 at 1/100, followed by conjugation to the secondary antibody and DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD130 (gp130) antibody (ab202850)Immunohistochemical analysis of formalin-fixed paraffin-embedded human endometrial cancer tissue labeling CD130 (gp130) with ab202850 at 1/100, followed by conjugation to the secondary antibody and DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD130 (gp130) antibody (ab202850)Paraformaldehyde-fixed, paraffin-embedded human colon carcinoma; antigen retrieval by boiling in sodium citrate buffer (0.01M, pH 6.0) for 15min; blocking buffer (normal goat serum) at 37°C for 20min; antibody incubation with ab202850 at 1/300 overnight at 4°C. A goat anti-rabbit IgG, FITC-conjugated secondary antibody was used for 40 mins at 37°C. DAPI (5µg/ml, blue) was used for nuclei staining.
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Flow Cytometry - Anti-CD130 (gp130) antibody (ab202850)Human Raji cells probed with ab202850 (green) at 1/100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
