Anti-NDUFB4 antibody [EPR16240] (ab192243)
![Anti-NDUFB4 antibody [EPR16240] (ab192243)](https://www.abcam.com/ps/products/192/ab192243/Images/ab192243-229899-anti-ndufb4-antibody-epr16240-western-blot.jpg "Anti-NDUFB4 antibody [EPR16240] (ab192243)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16240] to NDUFB4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-NDUFB4 antibody [EPR16240]
See all NDUFB4 primary antibodies -
Description
Rabbit monoclonal [EPR16240] to NDUFB4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseHumanWB RatHuman
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Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal heart, HepG2, Human cerebellum and Rat kidney lysates; Human and Mouse kidney tissues; A549 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16240 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NDUFB4 antibody [EPR16240] (ab192243)Anti-NDUFB4 antibody [EPR16240] (ab192243) at 1/20000 dilution + Human fetal heart lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 15 kDaBlocking/Dilution buffer: 5% NFDM /TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB4 antibody [EPR16240] (ab192243)](https://www.abcam.com/ps/products/192/ab192243/Images/ab192243-229893-anti-ndufb4-antibody-epr16240-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB4 antibody [EPR16240] (ab192243)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFB4 with ab192243 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
(inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-NDUFB4 antibody [EPR16240] (ab192243)](https://www.abcam.com/ps/products/192/ab192243/Images/ab192243-229897-anti-ndufb4-antibody-epr16240-western-blot.jpg)
Western blot - Anti-NDUFB4 antibody [EPR16240] (ab192243)All lanes : Anti-NDUFB4 antibody [EPR16240] (ab192243) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Human cerebellum lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 15 kDaBlocking/Dilution buffer: 5% NFDM /TBST.
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![Western blot - Anti-NDUFB4 antibody [EPR16240] (ab192243)](https://www.abcam.com/ps/products/192/ab192243/Images/ab192243-229894-anti-ndufb4-antibody-epr16240-western-blot.jpg)
Western blot - Anti-NDUFB4 antibody [EPR16240] (ab192243)Anti-NDUFB4 antibody [EPR16240] (ab192243) at 1/5000 dilution + Rat kidney lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 15 kDaBlocking/Dilution buffer: 5% NFDM /TBST.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB4 antibody [EPR16240] (ab192243)](https://www.abcam.com/ps/products/192/ab192243/Images/ab192243-229892-anti-ndufb4-antibody-epr16240-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB4 antibody [EPR16240] (ab192243)Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling NDUFB4 with ab192243 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
(inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-NDUFB4 antibody [EPR16240] (ab192243)](https://www.abcam.com/ps/products/192/ab192243/Images/ab192243-229891-anti-ndufb4-antibody-epr16240-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NDUFB4 antibody [EPR16240] (ab192243)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized A549 cells labeling NDUFB4 with ab192243 at 1/50 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody (ab150078) at 1/500 dilution. Nuclear counter stain Dapi (blue).
The two negative controls are anti-NDUFB4 at 1/50 dilution followed by (Goat anti mouse IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.
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![Anti-NDUFB4 antibody [EPR16240] (ab192243)](https://www.abcam.com/ps/products/192/ab192243/Images/ab192243-7-benefits-of-recombinant-antibodies.png)
Anti-NDUFB4 antibody [EPR16240] (ab192243)
