Anti-NDUFB4 antibody [17G3D9E12] (ab110243)
Key features and details
- Mouse monoclonal [17G3D9E12] to NDUFB4
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
- Reacts with: Rat, Cow, Human
- Isotype: IgG1
Overview
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Product name
Anti-NDUFB4 antibody [17G3D9E12]
See all NDUFB4 primary antibodies -
Description
Mouse monoclonal [17G3D9E12] to NDUFB4 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human -
Immunogen
Full length protein. This information is considered to be commercially sensitive.
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Positive control
- Isolated mitochondria from Human heart. HeLa cels. Cultured Human fibroblasts.
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General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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Purity
Proprietary Purification -
Purification notes
Near homogeneity as judged by SDS-PAGE (>95%). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
17G3D9E12 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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All lanes : Anti-NDUFB4 antibody [17G3D9E12] (ab110243) at 0.5 µg/ml
Lane 1 : Isolated mitochondria from Human heart at 5 µg
Lane 2 : Isolated mitochondria from cow heart at 1 µg
Lane 3 : Isolated mitochondria from rat heart at 10 µg
Predicted band size: 15 kDa
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Mitochondrial localization of complex I visualized by immunocytochemistry using aab110243.
Cultured Human fibroblasts were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110243 at 15µg/ml, followed by a fluorescent goat-anti-mouse IgG. -
HeLa cells were stained with 1 µg/mL ab110243 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
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Immunohistochemistical detection of Anti-NDUFB4 antibody [17G3D9E12] (ab110243; 1/100, incubated for 1hr) on formaldehyde fixed human cerebellum sections. Antigen retrieval step: Heat mediated. Permeabilization: No. ab110243 was used to investigate respiratory deficiency in 5µm cut sections of FFPE human cerebellum. Sections were cut and mounted on to glass adhesion slides for staining. The antibody stained specifically, identifying both normal neurons as well as those exhibiting respiratory deficiency. Non-specific background staining was not evident, and positive staining was crisp and punctate. Detection - Menarini Diagnostics X-Cell Plus HRP-Polymer Kit. SigmaFast DAB tablets were used for the final stage of staining.