Anti-Insulin degrading enzyme / IDE antibody (ab32216)
Key features and details
- Rabbit polyclonal to Insulin degrading enzyme / IDE
- Suitable for: WB, IHC-FoFr
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Insulin degrading enzyme / IDE antibody
See all Insulin degrading enzyme / IDE primary antibodies -
Description
Rabbit polyclonal to Insulin degrading enzyme / IDE -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-FoFr RatWB MouseHuman
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Immunogen
Synthetic peptide corresponding to Human Insulin degrading enzyme/ IDE aa 950 to the C-terminus.
(Peptide available asab32215)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32216 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species IHC-FoFr RatWB MouseHumanAll applications CowCatDogApplication Abreviews Notes WB (2) Use a concentration of 1 µg/ml. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).Can be blocked with Human Insulin degrading enzyme / IDE peptide (ab32215).IHC-FoFr (2) 1/100.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).Can be blocked with Human Insulin degrading enzyme / IDE peptide (ab32215).IHC-FoFr
1/100.Target
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Function
Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia. -
Sequence similarities
Belongs to the peptidase M16 family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Cytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform. - Information by UniProt
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Database links
- Entrez Gene: 523752 Cow
- Entrez Gene: 477768 Dog
- Entrez Gene: 3416 Human
- Entrez Gene: 15925 Mouse
- Entrez Gene: 25700 Rat
- Omim: 146680 Human
- SwissProt: Q24K02 Cow
- SwissProt: P14735 Human
see all -
Alternative names
- Abeta-degrading protease antibody
- FLJ35968 antibody
- Ide antibody
see all
Images
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Western blot - Anti-Insulin degrading enzyme / IDE antibody (ab32216)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Insulin degrading enzyme / IDE knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32216 observed at 120 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32216 was shown to specifically react with Insulin degrading enzyme / IDE in wild-type HAP1 cells. No band was observed when Insulin degrading enzyme / IDE knockout samples were examined. Wild-type and Insulin degrading enzyme / IDE knockout samples were subjected to SDS-PAGE. ab32216 and ab8245 (loading control to GAPDH) were diluted at 1μg/ml and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Insulin degrading enzyme / IDE antibody (ab32216)This image is courtesy of Sophie Pezet, CNRS, Paris, FranceImmunofluorescent staining for Insulin degrading enzyme/IDE in rat brain rat hippocampus using Rabbit polyclonal to Insulin degrading enzyme/IDE (ab32216). . The staining is located in the neuronal soma and is finely punctuated. The picture was acquired using the X20 objective. Protocol details: Rats were intracardially perfused with 4% paraformaldehyde. Whole brain tissue was post-fixed overnight in the same fixative, and cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut by cryostat for use in fre floating IHC. Primary antibody ab32216 was incubated overnight at 1/100 at room temperature. Secondary antibody Alexa fluor 488 1/1000 was incubated for 2 hours at room temperature. -
Western blot - Anti-Insulin degrading enzyme / IDE antibody (ab32216)All lanes : Anti-Insulin degrading enzyme / IDE antibody (ab32216) at 1 µg/ml
Lane 1 : Mouse Brain at 20 µg/ml
Lane 2 : Brain (Rat) Whole Cell Lysate - normal tissue at 20 µg
Lane 3 : Mouse Hippocampus Lysate at 20 µg
Lane 4 : Rat Hippocampus Lysate at 20 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 118 kDa
Observed band size: 118 kDa
Protocols
Datasheets and documents
References (50)
ab32216 has been referenced in 50 publications.
- Gee MS et al. A selective p38a/ß MAPK inhibitor alleviates neuropathology and cognitive impairment, and modulates microglia function in 5XFAD mouse. Alzheimers Res Ther 12:45 (2020). PubMed: 32317025
- Su W et al. Deletion of the Capn1 Gene Results in Alterations in Signaling Pathways Related to Alzheimer's Disease, Protein Quality Control and Synaptic Plasticity in Mouse Brain. Front Genet 11:334 (2020). PubMed: 32328086
- Khan MI et al. Green Tea Seed Isolated Theasaponin E1 Ameliorates AD Promoting Neurotoxic Pathogenesis by Attenuating Aß Peptide Levels in SweAPP N2a Cells. Molecules 25:N/A (2020). PubMed: 32429462
- Kurauti MA et al. Hyperinsulinemia is associated with increasing insulin secretion but not with decreasing insulin clearance in an age-related metabolic dysfunction mice model. J Cell Physiol 234:9802-9809 (2019). PubMed: 30370604
- Zhang R et al. Aquaporin 4 deletion exacerbates brain impairments in a mouse model of chronic sleep disruption. CNS Neurosci Ther N/A:N/A (2019). PubMed: 31364823
Images
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Western blot - Anti-Insulin degrading enzyme / IDE antibody (ab32216)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Insulin degrading enzyme / IDE knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32216 observed at 120 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32216 was shown to specifically react with Insulin degrading enzyme / IDE in wild-type HAP1 cells. No band was observed when Insulin degrading enzyme / IDE knockout samples were examined. Wild-type and Insulin degrading enzyme / IDE knockout samples were subjected to SDS-PAGE. ab32216 and ab8245 (loading control to GAPDH) were diluted at 1μg/ml and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Insulin degrading enzyme / IDE antibody (ab32216) This image is courtesy of Sophie Pezet, CNRS, Paris, FranceImmunofluorescent staining for Insulin degrading enzyme/IDE in rat brain rat hippocampus using Rabbit polyclonal to Insulin degrading enzyme/IDE (ab32216). . The staining is located in the neuronal soma and is finely punctuated. The picture was acquired using the X20 objective. Protocol details: Rats were intracardially perfused with 4% paraformaldehyde. Whole brain tissue was post-fixed overnight in the same fixative, and cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut by cryostat for use in fre floating IHC. Primary antibody ab32216 was incubated overnight at 1/100 at room temperature. Secondary antibody Alexa fluor 488 1/1000 was incubated for 2 hours at room temperature.
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Western blot - Anti-Insulin degrading enzyme / IDE antibody (ab32216)All lanes : Anti-Insulin degrading enzyme / IDE antibody (ab32216) at 1 µg/ml
Lane 1 : Mouse Brain at 20 µg/ml
Lane 2 : Brain (Rat) Whole Cell Lysate - normal tissue at 20 µg
Lane 3 : Mouse Hippocampus Lysate at 20 µg
Lane 4 : Rat Hippocampus Lysate at 20 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 118 kDa
Observed band size: 118 kDa
