Anti-Insulin degrading enzyme / IDE antibody (ab228720)
Key features and details
- Rabbit polyclonal to Insulin degrading enzyme / IDE
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-Insulin degrading enzyme / IDE antibody
See all Insulin degrading enzyme / IDE primary antibodies -
Description
Rabbit polyclonal to Insulin degrading enzyme / IDE -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog, Zebrafish, Rhesus monkey
-
Immunogen
Recombinant fragment within Human Insulin degrading enzyme/ IDE (internal sequence). The exact sequence is proprietary.
Database link: P14735 -
Positive control
- WB: HeLa cell extract; Mouse and rat brain extracts; A431, NCI-H1299, HeLa and HepG2 whole cell lysates. ICC/IF: HeLa cells. IHC-P: U87 xenograft tissue; Rat brain stem tissue.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Insulin degrading enzyme / IDE antibody (ab228720)All lanes : Anti-Insulin degrading enzyme / IDE antibody (ab228720) at 1/2500 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : Insulin degrading enzyme / IDE Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 118 kDa
-
Western blot - Anti-Insulin degrading enzyme / IDE antibody (ab228720)All lanes : Anti-Insulin degrading enzyme / IDE antibody (ab228720) at 1/2500 dilution
Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 2 : Insulin degrading enzyme / IDE knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 118 kDa7.5% SDS-PAGE gel.
-
Immunocytochemistry/ Immunofluorescence - Anti-Insulin degrading enzyme / IDE antibody (ab228720)Ice-cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Insulin degrading enzyme / IDE (green) using ab228720 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin degrading enzyme / IDE antibody (ab228720)Paraffin-embedded U87 xenograft tissue stained for Insulin degrading enzyme / IDE using ab228720 at 1/500 dilution in immunohistochemical analysis.
-
Western blot - Anti-Insulin degrading enzyme / IDE antibody (ab228720)All lanes : Anti-Insulin degrading enzyme / IDE antibody (ab228720) at 1/1000 dilution
Lane 1 : Mouse brain extract
Lane 2 : Rat brain extract
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 118 kDa7.5% SDS-PAGE gel.
-
Western blot - Anti-Insulin degrading enzyme / IDE antibody (ab228720)All lanes : Anti-Insulin degrading enzyme / IDE antibody (ab228720) at 1/10000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 118 kDa7.5% SDS-PAGE gel.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin degrading enzyme / IDE antibody (ab228720)Paraffin-embedded rat brain stem tissue stained for Insulin degrading enzyme / IDE using ab228720 at 1/500 dilution in immunohistochemical analysis.
