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Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6098(2)] to Insulin degrading enzyme / IDE
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)]
    See all Insulin degrading enzyme / IDE primary antibodies

  • Description

    Rabbit monoclonal [EPR6098(2)] to Insulin degrading enzyme / IDE

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Insulin degrading enzyme/ IDE aa 50-150. The exact sequence is proprietary.
    Database link: P14735

  • Positive control

    • HeLa, HepG2, A375, and K562 cell lysates, Human colon tissue

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)
    Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: IDE knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 cell lysate (20 µg)
    Lane 4: HepG2 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab133561 observed at 120 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab133561 was shown to specifically react with IDE in wild-type HAP1 cells. No band was observed when IDE knockout samples were examined. Wild-type and IDE knockout samples were subjected to SDS-PAGE. ab133561 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)
    Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)
    All lanes : Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : A375 cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 118 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)

    Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Insulin degrading enzyme / IDE with ab133561 antibody at a dilution of 1/50.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)
    OI-RD Scanning - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)
    Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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