Antibodies, Proteins, Kits and Reagents for Life Science

355 142 ₸ Product size

100 µl 355 142 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6098(2)] to Insulin degrading enzyme / IDE
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Product name

Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)]
See all Insulin degrading enzyme / IDE primary antibodies
Description

Rabbit monoclonal [EPR6098(2)] to Insulin degrading enzyme / IDE
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human Insulin degrading enzyme/ IDE aa 50-150. The exact sequence is proprietary.
Database link: P14735
Positive control
- HeLa, HepG2, A375, and K562 cell lysates, Human colon tissue
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 2.31 x 10 ^-10 M
Learn more about K_D
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR6098(2)
Isotype

IgG
Research areas

Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: IDE knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab133561 observed at 120 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab133561 was shown to specifically react with IDE in wild-type HAP1 cells. No band was observed when IDE knockout samples were examined. Wild-type and IDE knockout samples were subjected to SDS-PAGE. ab133561 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)

All lanes : Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A375 cell lysate
Lane 4 : K562 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 118 kDa
Observed band size: 120 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)

Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Insulin degrading enzyme / IDE with ab133561 antibody at a dilution of 1/50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
OI-RD Scanning - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)

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