Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] - BSA and Azide free (ab233020)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6098(2)] to Insulin degrading enzyme / IDE - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] - BSA and Azide free
    See all Insulin degrading enzyme / IDE primary antibodies

  • Description

    Rabbit monoclonal [EPR6098(2)] to Insulin degrading enzyme / IDE - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2 and K562 cell lysates. Wild-type HAP1 cell lysate.

  • General notes

    Ab233020 is the carrier-free version of ab133561. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab233020 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] - BSA and Azide free (ab233020)
    Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] - BSA and Azide free (ab233020)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: IDE knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 cell lysate (20 µg)
    Lane 4: HepG2 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab133561 observed at 120 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab133561 was shown to specifically react with IDE in wild-type HAP1 cells. No band was observed when IDE knockout samples were examined. Wild-type and IDE knockout samples were subjected to SDS-PAGE. ab133561 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133561).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] - BSA and Azide free (ab233020)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] - BSA and Azide free (ab233020)

    Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Insulin degrading enzyme / IDE with ab133561 antibody at a dilution of 1/50.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133561).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] - BSA and Azide free (ab233020)
    OI-RD Scanning - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] - BSA and Azide free (ab233020)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133561).

  • Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] - BSA and Azide free (ab233020)
    Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] - BSA and Azide free (ab233020)

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