Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Kv1.1 potassium channel antibody [K20/78] - BSA and Azide free (ab254277)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [K20/78] to Kv1.1 potassium channel - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Kv1.1 potassium channel antibody [K20/78] - BSA and Azide free
    See all Kv1.1 potassium channel primary antibodies

  • Description

    Mouse monoclonal [K20/78] to Kv1.1 potassium channel - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC or IHC-Fr

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat hippocampus tissue lysate. IHC-P: Mouse hippocampus tissue. Rat pancreas and hippocampus tissue.

  • General notes

    ab254277 is the carrier-free version of ab252537.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Kv1.1 potassium channel antibody [K20/78] - BSA and Azide free (ab254277)
    Western blot - Anti-Kv1.1 potassium channel antibody [K20/78] - BSA and Azide free (ab254277)
    All lanes : Anti-Kv1.1 potassium channel antibody [K20/78] (ab252537) at 1/1000 dilution

    Lane 1 : Mouse hippocampus tissue lysate
    Lane 2 : Rat hippocampus tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 56 kDa


    Exposure time: 37 seconds


    This data was produced using the same clone but in a different formulation, ab252537.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 31978607, 20805574, 24498366).
    Samples are non-boiled as boiling may cause protein aggregates.

    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv1.1 potassium channel antibody [K20/78] - BSA and Azide free (ab254277)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv1.1 potassium channel antibody [K20/78] - BSA and Azide free (ab254277)

    This data was produced using the same clone but in a different formulation, ab252537.

    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling Kv1.1 potassium channel with ab252537 at 1/1000 followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on rat hippocampus. The section was incubated with ab252537 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv1.1 potassium channel antibody [K20/78] - BSA and Azide free (ab254277)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv1.1 potassium channel antibody [K20/78] - BSA and Azide free (ab254277)

    This data was produced using the same clone but in a different formulation, ab252537.

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling Kv1.1 potassium channel with ab252537 at 1/1000 followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on rat pancreas islet. The section was incubated with ab252537 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv1.1 potassium channel antibody [K20/78] - BSA and Azide free (ab254277)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv1.1 potassium channel antibody [K20/78] - BSA and Azide free (ab254277)

    This data was produced using the same clone but in a different formulation, ab252537.

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling Kv1.1 potassium channel with ab252537 at 1/1000 followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on mouse hippocampus. The section was incubated with ab252537 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Anti-Kv1.1 potassium channel antibody [K20/78] - BSA and Azide free (ab254277)
    Anti-Kv1.1 potassium channel antibody [K20/78] - BSA and Azide free (ab254277)

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