Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free (ab247442)
![Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free (ab247442)](https://www.abcam.com/ps/products/247/ab247442/Images/ab247442-410635-anti-fak-phospho-y576-antibody-ep1897y-bsa-and-azide-free-western-blot.jpg "Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free (ab247442)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1897Y] to FAK (phospho Y576) - BSA and Azide free
- Suitable for: Flow Cyt, Dot blot, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free
See all FAK primary antibodies -
Description
Rabbit monoclonal [EP1897Y] to FAK (phospho Y576) - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody detects FAK phosphorylated at Y576.
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Tested applications
Suitable for: Flow Cyt, Dot blot, WBmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab247442 is the carrier-free version of ab76120. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247442 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EP1897Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free (ab247442)All lanes : Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120) at 1/1000 dilution (Purified)
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa treated with 50nM pervanadate for 60 minutes whole cell lysate
Lane 3 : HeLa treated with 50nM pervanadate for 60 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 119 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?This data was developed using ab76120, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Flow Cytometry - Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free (ab247442)](https://www.abcam.com/ps/products/247/ab247442/Images/ab247442-4-anti-fak-phospho-y576-antibody-ep1897y-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free (ab247442)This data was developed using ab76120, the same antibody clone in a different buffer formulation.Flow cytometry analysis of HeLa cells treated with 1mM Pervanadate for 30mins cells labeling FAK (phospho Y576)with ab76120 at 1/400 dilution (0.1µg) (red). Cells were fixed with 4% paraformaldehyde . Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730 / Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). -
![Dot Blot - Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free (ab247442)](https://www.abcam.com/ps/products/247/ab247442/Images/ab247442-3-anti-fak-phospho-y576-antibody-ep1897y-bsa-and-azide-free-dot-blot.jpg)
Dot Blot - Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free (ab247442)This data was developed using ab76120, the same antibody clone in a different buffer formulation.Dot blot analysis of FAK (phospho Y576) phospho peptide (Lane 1) and FAK non-phospho peptide (Lane 2) labelling FAK (phospho Y576) phospho peptide with ab76120 at a dilution of 1/1000 dilution (0.441ug/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST . -
![Western blot - Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free (ab247442)](https://www.abcam.com/ps/products/247/ab247442/Images/ab247442-403221-anti-fak-phospho-y576-antibody-ep1897y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free (ab247442)All lanes : Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 2 : NIH/3T3 treated with 10mM pervanadate for 20 minutes whole cell lysate
Lane 3 : NIH/3T3 treated with 10mM pervanadate for 20 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 119 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?This data was developed using ab76120, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free (ab247442)](https://www.abcam.com/ps/products/247/ab247442/Images/ab247442-397946-anti-fak-phospho-y576-antibody-ep1897y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free (ab247442)All lanes : Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120) at 1/50000 dilution
Lane 1 : 3T3 cell lysates untreated
Lane 2 : 3T3 cell lysates treated with pervanadate.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 119 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?This data was developed using ab76120, the same antibody clone in a different buffer formulation.
-
![Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free (ab247442)](https://www.abcam.com/ps/products/247/ab247442/Images/ab247442-6-benefits-of-recombinant-antibodies.png)
Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free (ab247442)