Anti-Integrin beta 3 (phospho Y773) antibody (ab38460)
Key features and details
- Rabbit polyclonal to Integrin beta 3 (phospho Y773)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Integrin beta 3 (phospho Y773) antibody
See all Integrin beta 3 primary antibodies -
Description
Rabbit polyclonal to Integrin beta 3 (phospho Y773) -
Host species
Rabbit -
Specificity
ab38460 recognises endogenous levels of Integrin beta 3 only when phosphorylated at tyrosine 773. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic phosphopeptide derived from human Integrin beta 3 around the phosphorylation site of tyrosine 773 (PLYpKE)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Integrin beta 3 (phospho Y773) antibody (ab38460)All lanes : Anti-Integrin beta 3 (phospho Y773) antibody (ab38460) at 1/500 dilution
Lane 1 : Untreated extracts from HL-60 cells
Lane 2 : Hydrogen peroxide treated extracts from HL-60 cells
Lysates/proteins at 30 µg per lane.
Predicted band size: 87 kDa
Observed band size: >118 kDa why is the actual band size different from the predicted?
Lanes can be loaded with 5-30µg of total protein. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 (phospho Y773) antibody (ab38460)Immunohistochemical analysis of Integrin beta 3 (phospho Y773) expression in paraffin embedded human breast carcinoma tissue sections using 1/50 ab38460. Left: untreated sample. Right: Sample pretreated with phosphopeptide (negative control).
