Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Integrin beta 3 antibody [EPR2342] (ab119992)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2342] to Integrin beta 3
Suitable for: WB, Flow Cyt
Reacts with: Mouse, Human

Product name

Anti-Integrin beta 3 antibody [EPR2342]
See all Integrin beta 3 primary antibodies
Description

Rabbit monoclonal [EPR2342] to Integrin beta 3
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: U-87MG,HEL,C2C12,THP-1 treated with TPA, HUVEC, Human spleen and Human plasma lysates. Flow Cyt: HUVEC cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR2342
Isotype

IgG
Research areas

Western blot - Anti-Integrin beta 3 antibody [EPR2342] (ab119992)

All lanes : Anti-Integrin beta 3 antibody [EPR2342] (ab119992) at 1/500 dilution

Lane 1 : U-87MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates with 5% NFDM/TBST
Lane 2 : HEL (Human Erythroleukemia erythroblast) whole cell lysates with 5% NFDM/TBST
Lane 3 : LnCaP (Human prostate carcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
Lane 5 : HUVEC (Human umbilical vein endothelial cell) whole cell lysates with 5% NFDM/TBST
Lane 6 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
Lane 7 : C2C12 (Mouse myoblasts myoblast) whole cell lysates with 5% NFDM/TBST
Lane 8 : Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates with 5% NFDM/TBST

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 87 kDa
Observed band size: 110 kDa
why is the actual band size different from the predicted?

LnCaP, HeLa and PC-3 are low expression controls.

Exposure:

1,2 lane: 10 s;

3-8 lanes: 3 min
Flow Cytometry - Anti-Integrin beta 3 antibody [EPR2342] (ab119992)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HUVEC (Human umbilical vein endothelial cell) cell line labeling Integrin beta 3 with ab119992 at 1/20 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-Integrin beta 3 antibody [EPR2342] (ab119992)

All lanes : Anti-Integrin beta 3 antibody [EPR2342] (ab119992) at 1/1000 dilution

Lane 1 : TPA treated THP1 cell lysate
Lane 2 : HUVEC cell lysate
Lane 3 : Human spleen lysate
Lane 4 : Human plasma lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 87 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Anti-Integrin beta 3 antibody [EPR2342] (ab119992)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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