Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 500 µl 1 340 ₸

Order now and get it on Wednesday March 17, 2021

Anti-Integrin beta 3 antibody [SP324] (ab227702)

Key features and details

Rabbit monoclonal [SP324] to Integrin beta 3
Suitable for: WB, IHC-P
Reacts with: Mouse, Human
Isotype: IgG

Product name

Anti-Integrin beta 3 antibody [SP324]
See all Integrin beta 3 primary antibodies
Description

Rabbit monoclonal [SP324] to Integrin beta 3
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to Human Integrin beta 3 (internal sequence).
Database link: P05106
Positive control
- WB: HUVEC cell lysate. IHC-P: Human bone marrow and spleen tissues.
General notes

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
Purity

Immunogen affinity purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP324
Isotype

IgG
Research areas

Western blot - Anti-Integrin beta 3 antibody [SP324] (ab227702)

All lanes : Anti-Integrin beta 3 antibody [SP324] (ab227702) at 1/500 dilution

Lane 1 : U-87MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates
Lane 2 : HEL (Human Erythroleukemia erythroblast) whole cell lysates
Lane 3 : LnCaP (Human prostate carcinoma epithelial cell) whole cell lysates
Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 5 : HUVEC (Human umbilical vein endothelial cell) whole cell lysates
Lane 6 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
Lane 7 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
Lane 8 : Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
Lane 9 : Mouse brain lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 87 kDa
Observed band size: 110 kDa
why is the actual band size different from the predicted?

Exposure time: 10 seconds

LnCaP, HeLa and PC-3 are low expression controls. This product doesn’t react with Ms
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [SP324] (ab227702)

Formalin-fixed, paraffin-embedded human spleen tissue stained for Integrin beta 3 using ab227702 at 1/100 dilution in immunohistochemical analysis.
Western blot - Anti-Integrin beta 3 antibody [SP324] (ab227702)

Anti-Integrin beta 3 antibody [SP324] (ab227702) at 1/100 dilution + HUVEC (human umbilical vein endothelial cell line) cell lysate

Predicted band size: 87 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [SP324] (ab227702)

Formalin-fixed, paraffin-embedded human bone marrow tissue stained for Integrin beta 3 using ab227702 at 1/100 dilution in immunohistochemical analysis.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com