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Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins

Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244)

Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1832Y] to FAK (phospho Y576 + Y577)
  • Suitable for: WB, Dot blot
  • Reacts with: Mouse

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Overview

  • Product name

    Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y]
    See all FAK primary antibodies
  • Description

    Rabbit monoclonal [EP1832Y] to FAK (phospho Y576 + Y577)
  • Host species

    Rabbit
  • Specificity

    Detects FAK phosphorylated at Y576/577.
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding Tyrosine 576/577 of human FAK.

  • Positive control

    • NIH-3T3 lysate treated with pervanadate.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP1832Y
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Integrins
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • FAK / PYK
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Focal Adhesions
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Tyrosine kinases
    • FAK family

Images

  • Dot Blot - Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244)
    Dot Blot - Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244)

    Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244) Dot Blot. Primary ab dilution 1:1000,  Secondary ab description and code (ab id), Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), Secondary ab dilution 1:100,000, Blocking buffer and concentration 5% NFDM/TBST, Diluting buffer and concentration 5% NFDM /TBST, Lane 1:FAK (pY576+pY577) phospho peptide, Lane 2:FAK (pY576) phospho peptide, Lane 3:FAK (pY577) phospho peptide, Lane 4:FAK non-phospho peptide, Exposure time 3 minutes. Note: antibody used for ab76244 is purified batch.

  • Western blot - Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244)
    Western blot - Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244)
    All lanes : Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244) at 1/50000 dilution

    Lane 1 : NIH-3T3 cell lysates, untreated
    Lane 2 : NIH-3T3 cell lysates treated with pervanadate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/1000 dilution

    Predicted band size: 119 kDa
    Observed band size: 125 kDa
    why is the actual band size different from the predicted?

  • Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244)
    Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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