Anti-FAK (phospho S732) antibody (ab4792)
Key features and details
- Rabbit polyclonal to FAK (phospho S732)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-FAK (phospho S732) antibody
See all FAK primary antibodies -
Description
Rabbit polyclonal to FAK (phospho S732) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chicken, Xenopus laevis
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Immunogen
Synthetic peptide (Human) derived from the region of human Focal Adhesion Kinase that contains serine 732. The sequence is conserved in mouse, rat and chicken.
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General notes
Focal Adhesion Kinase is a 125 kDa non-receptor protein tyrosine kinase that plays a key role in signalling by growth factors, extracellular matrix and stress signals. Indeed, Focal Adhesion Kinase plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Cyclin-dependent kinase-5 (Cdk5), a serine/threonine kinase and an important regulator in brain development, has been found to be essential for phosphorylation at serine 732. In the context of the Focal Adhesion Kinase-Related Non-Kinase (FRNK), the c-terminal non-kinase domain of Focal Adhesion Kinase that acts as a dominant negative inhibitor of Focal Adhesion Kinase signalling, other serine kinases appear to readily phosphorylate this same site. The functional significance of phosphorylation on serine 732 is yet to be determined.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 2.5% Glycerol, 0.1% BSA
BSA is IgG and protease free -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Focal Adhesion Kinase enzyme and (ii) a generic serine phosphorylated peptide to remove antibody that is reactive with phosphoserine, irrespective of the sequence. The final product is generated by affinity chromatography using a Focal Adhesion Kinase-derived peptide that is phosphorylated at serine 732. -
Primary antibody notes
Focal Adhesion Kinase is a 125 kDa non-receptor protein tyrosine kinase that plays a key role in signalling by growth factors, extracellular matrix and stress signals. Indeed, Focal Adhesion Kinase plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Cyclin-dependent kinase-5 (Cdk5), a serine/threonine kinase and an important regulator in brain development, has been found to be essential for phosphorylation at serine 732. In the context of the Focal Adhesion Kinase-Related Non-Kinase (FRNK), the c-terminal non-kinase domain of Focal Adhesion Kinase that acts as a dominant negative inhibitor of Focal Adhesion Kinase signalling, other serine kinases appear to readily phosphorylate this same site. The functional significance of phosphorylation on serine 732 is yet to be determined. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FAK (phospho S732) antibody (ab4792)All lanes : Anti-Metabotropic Glutamate Receptor 6/MGLUR6 antibody (ab10314)
Lane 1 : A549 without VEGF treatment.
Lane 2 : A549 with 5 ng/ml VEGF treatment for 5 mins.
Predicted band size: 125 kDa
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Western blot - Anti-FAK (phospho S732) antibody (ab4792)Peptide Competition: Extracts prepared from cdk5 +/- mouse brain lysates were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with FAK pan antibody (1), or 0.34
µ g/mL ab4792 antibody for two hours at room temperature in a 3% BSA TBST buffer, following prior incubation with: no peptide (2), the phosphopeptide corresponding to ab4792 (3), a generic phosphoserine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method.Phosphatase Stripping: Extracts prepared from cdk5 +/- mouse brain lysates were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was treated with lambda phosphatase, then incubated with either FAK pan antibody (
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Western blot - Anti-FAK (phospho S732) antibody (ab4792)Influence of cdk5: Extracts prepared from cdk5 +/- (1, 3) and cdk5 -/- (2, 4) mouse brain lysates were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was incubated with either FAK pan antibody (1, 2), or with 0.34µ g/mL ab4792 antibody (3, 4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that cdk5 is essential for phosphorylation of FAK at serine 732. Other kinases appear to phosphorylate FRNK on this site. Influence of cdk5: Extracts prepared from cdk5 +/- (1, 3) and cdk5 -/- (2, 4) mouse brain lysates were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was incubated with either FAK pan antibody (1, 2), or with 0.34 µg/mL ab4792 antibody (3, 4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were de
