Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1831Y] to FAK
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Product name

Anti-FAK antibody [EP1831Y]
See all FAK primary antibodies
Description

Rabbit monoclonal [EP1831Y] to FAK
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human FAK aa 300-400. The exact sequence is proprietary.
Positive control
- WB: HeLa, A431 and HEK-293T cell lysate. IHC-P: human tonsil tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP1831Y
Isotype

IgG
Research areas

Western blot - Anti-FAK antibody [EP1831Y] (ab76496)

All lanes : Anti-FAK antibody [EP1831Y] (ab76496) at 1/500 dilution

Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Wild-type HEK-293T cell lysate
Lane 4 : PTK2 knockout HEK-293T cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 119 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab76496 observed at 119 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab76496 was shown to react with FAK in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255421 (knockout cell lysate ab263766) was used. Wild-type and FAK knockout samples were subjected to SDS-PAGE. ab76496 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP1831Y] (ab76496)

Immunohistochemical staining of paraffin-embedded human tonsils using 1/100 ab76496

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-FAK antibody [EP1831Y] (ab76496)

Anti-FAK antibody [EP1831Y] (ab76496) at 1/500 dilution + HeLa cell lysate at 10 µg

Secondary
HRP labeled goat anti-rabbit at 1/1000 dilution

Predicted band size: 119 kDa
Anti-FAK antibody [EP1831Y] (ab76496)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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