Anti-FAK (phospho Y407) antibody (ab4814)
Key features and details
- Rabbit polyclonal to FAK (phospho Y407)
- Suitable for: WB
- Reacts with: Chicken
- Isotype: IgG
Overview
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Product name
Anti-FAK (phospho Y407) antibody
See all FAK primary antibodies -
Description
Rabbit polyclonal to FAK (phospho Y407) -
Host species
Rabbit -
Specificity
Phosphorylation site-specific antibody selective for the phosphorylated form of the Focal Adhesion Kinase enzyme containing a phosphate on tyrosine 407. The antibody has been shown to recognize Focal Adhesion Kinase (approximately 125 kDa) in chick embryo fibroblast cells plated on fibronectin. -
Tested Applications & Species
See all applications and species dataApplication Species WB Chicken
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Immunogen
Synthetic peptide derived from the region of Focal Adhesion Kinase that contains tyrosine 407. The sequence is conserved in human, mouse, rat, chicken and frog.
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General notes
Focal Adhesion Kinase is a non-receptor protein tyrosine kinase discovered as a substrate for Src and as a key element of integrin signalling. Focal Adhesion Kinase plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. The activity of the phosphorylation site pTyr407 is currently unknown.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Focal Adhesion Kinase enzyme and (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using a Focal Adhesion Kinase-derived peptide that is phosphorylated at tyrosine 407. -
Primary antibody notes
Focal Adhesion Kinase is a non-receptor protein tyrosine kinase discovered as a substrate for Src and as a key element of integrin signalling. Focal Adhesion Kinase plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. The activity of the phosphorylation site pTyr407 is currently unknown. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FAK (phospho Y407) antibody (ab4814)Cell extracts prepared from chick embryo fibroblasts expressing FAK and plated on fibronectin were resolved by SDS-PAGE on a 4-20% Tris-glycine gel. The proteins were then transferred to nitrocellulose. Membranes were incubated with 0.3
µ g/mL ab4814, following prior incubation in the absence (none) or presence of the peptide immunogen, the non-phosphopeptide corresponding to the FAK phosphopeptide, or a generic phosphotyrosine peptide. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. The data show that only the phosphopeptide corresponding to this site blocks the antibody signal, therefore demonstrating the specificity of ab4814 for this phosphorylated residue. Cell extracts prepared from chick embryo fibroblasts expressing FAK and plated on fibronectin were resolved by SDS-PAGE on a 4-20% Tris-glycine gel. The proteins were then transferred to
