Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FAK antibody [EP695Y] - Low endotoxin, Azide free (ab219363)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP695Y] to FAK - Low endotoxin, Azide free
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-FAK antibody [EP695Y] - Low endotoxin, Azide free
    See all FAK primary antibodies

  • Description

    Rabbit monoclonal [EP695Y] to FAK - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Specificity

    The mouse, rat and cow recommendation is based on the WB results. We do not guarantee IHC-P for mouse, rat and cow.

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NIH/3T3, Rat brain, HeLa, K-562. A431 and HEK-293T lysates IHC-P: human hepatocellular carcinoma

  • General notes

    ab219363 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-FAK antibody [EP695Y] - Low endotoxin, Azide free (ab219363)
    Western blot - Anti-FAK antibody [EP695Y] - Low endotoxin, Azide free (ab219363)
    All lanes : Anti-FAK antibody [EP695Y] (ab40794) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Wild-type HEK-293T cell lysate
    Lane 4 : PTK2 knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 119 kDa
    Observed band size: 119 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab40794).

    Lanes 1 - 4: Merged signal (red and green). Green - ab40794 observed at 119 kDa. Red - loading control, ab8245 observed at 37 kDa.

     ab40794 was shown to react with FAK in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255421 (knockout cell lysate ab263766) was used. Wild-type and FAK knockout samples were subjected to SDS-PAGE. ab40794 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] - Low endotoxin, Azide free (ab219363)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] - Low endotoxin, Azide free (ab219363)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular carcinoma tissue sections labeling FAK with purified ab40794 at 1:250 dilution (2.32 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] - Low endotoxin, Azide free (ab219363)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] - Low endotoxin, Azide free (ab219363)
    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using ab40794.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40794).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] - Low endotoxin, Azide free (ab219363)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] - Low endotoxin, Azide free (ab219363) This image is courtesy of an Abreview submitted by Carl Hobbs.
    The image shows FAK antibody (ab40794) in human spleen tissue. Clear cytoplasmic positivity in a subset of germinal centre cells. The there is intense positivity of the serum in the blood vessels. Endogenous peroxidases was blocked using 2% H2O2, for 15 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40794).

  • Anti-FAK antibody [EP695Y] - Low endotoxin, Azide free (ab219363)
    Anti-FAK antibody [EP695Y] - Low endotoxin, Azide free (ab219363)

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