Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 09, 2021

Anti-EWSR1/EWS antibody [EPR4647] (ab133288)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4647] to EWSR1/EWS
Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-EWSR1/EWS antibody [EPR4647]
See all EWSR1/EWS primary antibodies
Description

Rabbit monoclonal [EPR4647] to EWSR1/EWS
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Rat Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human EWSR1/EWS aa 100-200. The exact sequence is proprietary.
Positive control
- WB: HeLa, Jurkat, F9, K562, Mouse brain, and Rat brain lysates. IHC-P: human liver carcinoma, colon and colonic adenocarcinoma, mouse spleen, and rat liver tissues. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
General notes
This product was previously labelled as EWSR1

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4647
Isotype

IgG
Research areas

Flow Cytometry - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling EWSR1/EWS with purified ab133288 at 1:100 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling EWSR1/EWS with purified ab133288 at 1:500 dilution (1.79 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling EWSR1/EWS with purified ab133288 at 1:500 dilution (1.79 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue sections labeling EWSR1/EWS with purified ab133288 at 1:500 dilution (1.79 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue sections labeling EWSR1/EWS with purified ab133288 at 1:500 dilution (1.79 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)

All lanes : Anti-EWSR1/EWS antibody [EPR4647] (ab133288) at 1/5000 dilution (Purified)

Lane 1 : Mouse brain lysates
Lane 2 : Rat brain lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 68 kDa

The molecular weight observed is consistent with what has been described in PMID: 30354839
Western blot - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)

Anti-EWSR1/EWS antibody [EPR4647] (ab133288) at 1/10000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 68 kDa
Observed band size: 90 kDa
why is the actual band size different from the predicted?

The molecular weight observed is consistent with what has been described in PMID: 30354839
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling EWSR1/EWS with unpurified ab133288 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)

Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labelling EWSR1/EWS with unpurified ab133288 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)

All lanes : Anti-EWSR1/EWS antibody [EPR4647] (ab133288) at 1/10000 dilution

Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : F9 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-rabbit HRP at 1/2000 dilution

Predicted band size: 68 kDa
Anti-EWSR1/EWS antibody [EPR4647] (ab133288)

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