Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EWSR1/EWS antibody [EPR4647] (ab133288)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4647] to EWSR1/EWS
  • Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-EWSR1/EWS antibody [EPR4647]
    See all EWSR1/EWS primary antibodies

  • Description

    Rabbit monoclonal [EPR4647] to EWSR1/EWS

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human EWSR1/EWS aa 100-200. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, Jurkat, F9, K562, Mouse brain, and Rat brain lysates. IHC-P: human liver carcinoma, colon and colonic adenocarcinoma, mouse spleen, and rat liver tissues. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.

  • General notes

    This product was previously labelled as EWSR1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Flow Cytometry - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)
    Flow Cytometry - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling EWSR1/EWS with purified ab133288 at 1:100 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunocytochemistry/ Immunofluorescence - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)
    Immunocytochemistry/ Immunofluorescence - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)
    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling EWSR1/EWS with purified ab133288 at 1:500 dilution (1.79 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling EWSR1/EWS with purified ab133288 at 1:500 dilution (1.79 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue sections labeling EWSR1/EWS with purified ab133288 at 1:500 dilution (1.79 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue sections labeling EWSR1/EWS with purified ab133288 at 1:500 dilution (1.79 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)
    Western blot - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)
    All lanes : Anti-EWSR1/EWS antibody [EPR4647] (ab133288) at 1/5000 dilution (Purified)

    Lane 1 : Mouse brain lysates
    Lane 2 : Rat brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 68 kDa



    The molecular weight observed is consistent with what has been described in PMID: 30354839

  • Western blot - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)
    Western blot - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)
    Anti-EWSR1/EWS antibody [EPR4647] (ab133288) at 1/10000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 68 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?



    The molecular weight observed is consistent with what has been described in PMID: 30354839

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling EWSR1/EWS with unpurified ab133288 at 1/250 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)

    Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labelling EWSR1/EWS with unpurified ab133288 at 1/250 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)
    Western blot - Anti-EWSR1/EWS antibody [EPR4647] (ab133288)
    All lanes : Anti-EWSR1/EWS antibody [EPR4647] (ab133288) at 1/10000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : F9 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 68 kDa

  • Anti-EWSR1/EWS antibody [EPR4647] (ab133288)
    Anti-EWSR1/EWS antibody [EPR4647] (ab133288)

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