Anti-EWSR1/EWS antibody [EPR4648] (ab133318)
![Anti-EWSR1/EWS antibody [EPR4648] (ab133318)](https://www.abcam.com/ps/products/133/ab133318/Images/ab133318-146823-anti-ewsr1-antibody-epr4648-western-blot.jpg "Anti-EWSR1/EWS antibody [EPR4648] (ab133318)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4648] to EWSR1/EWS
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Overview
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Product name
Anti-EWSR1/EWS antibody [EPR4648]
See all EWSR1/EWS primary antibodies -
Description
Rabbit monoclonal [EPR4648] to EWSR1/EWS -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide within Human EWSR1/EWS aa 300-400. The exact sequence is proprietary.
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Positive control
- K562, HeLa, Jurkat and F9 cell lysates; Human colon and spleen tissues.
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General notes
This product was previously labelled as EWSR1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4648 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EWSR1/EWS antibody [EPR4648] (ab133318)All lanes : Anti-EWSR1/EWS antibody [EPR4648] (ab133318) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : F9 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 68 kDaSecondary antibody - anti-rabbit HRP (ab6721)
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody [EPR4648] (ab133318)](https://www.abcam.com/ps/products/133/ab133318/Images/ab133318-146822-anti-ewsr1-antibody-epr4648-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody [EPR4648] (ab133318)Immunohistochemical analysis of Paraffin Embedded Human colon tissue labelling EWSR1/EWS with ab133318 at 1/500 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody [EPR4648] (ab133318)](https://www.abcam.com/ps/products/133/ab133318/Images/ab133318-146821-anti-ewsr1-antibody-epr4648-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EWSR1/EWS antibody [EPR4648] (ab133318)Immunohistochemical analysis of Paraffin Embedded Human spleen tissue labelling EWSR1/EWS with ab133318 at 1/500 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-EWSR1/EWS antibody [EPR4648] (ab133318)](https://www.abcam.com/ps/products/133/ab133318/Images/ab133318-4-benefits-of-recombinant-antibodies.png)
Anti-EWSR1/EWS antibody [EPR4648] (ab133318)
