Antibodies, Proteins, Kits and Reagents for Life Science

341 740 ₸ Product size

100 µl 341 740 ₸ 10 µl 137 366 ₸

Order now and get it on Tuesday March 09, 2021

Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12722(B)] to DNA Polymerase beta
Suitable for: ICC/IF, IP, IHC-P, WB
Knockout validated
Reacts with: Human

Product name

Anti-DNA Polymerase beta antibody [EPR12722(B)]
See all DNA Polymerase beta primary antibodies
Description

Rabbit monoclonal [EPR12722(B)] to DNA Polymerase beta
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DNA Polymerase beta aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P06746
Positive control
- WB: HeLa, Jurkat, K562 and A431 cell lysates. IHC-P: Human testis and uterus tissues. ICC/IF: A431 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12722(B)
Isotype

IgG
Research areas

Western blot - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)

All lanes : Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197) at 1/500 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : POLB knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 38 kDa
Observed band size: 38 kDa

anes 1-3: Merged signal (red and green). Green - ab175197 observed at 38 kDa. Red - loading control, ab7291 observed at 50 kDa.

ab175197 Anti-DNA Polymerase beta antibody [EPR12722(B)] was shown to specifically react with DNA Polymerase beta in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261748 (knockout cell lysate ab257096) was used. Wild-type and DNA Polymerase beta knockout samples were subjected to SDS-PAGE. ab175197 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti- Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti- Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)

All lanes : Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197) at 1/1000 dilution

Lane 1 : HeLa cell lysates
Lane 2 : Jurkat cell lysates
Lane 3 : K562 cell lysates
Lane 4 : A431 cell lysates

Lysates/proteins at 10 µg per lane.

Predicted band size: 38 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling DNA Polymerase beta with ab175197 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)

Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling DNA Polymerase beta with ab175197 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)

Immunofluorescence analysis of A431 cells labeling DNA Polymerase beta with ab175197 at a 1/50 dilution.
Immunoprecipitation - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)

Western blot analysis on immunoprecipitation pellet from K562 cell lysate labeling DNA Polymerase beta with ab175197 at 1/10 dilution.
Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)

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