Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12722(B)] to DNA Polymerase beta
  • Suitable for: ICC/IF, IP, IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-DNA Polymerase beta antibody [EPR12722(B)]
    See all DNA Polymerase beta primary antibodies

  • Description

    Rabbit monoclonal [EPR12722(B)] to DNA Polymerase beta

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DNA Polymerase beta aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P06746

  • Positive control

    • WB: HeLa, Jurkat, K562 and A431 cell lysates. IHC-P: Human testis and uterus tissues. ICC/IF: A431 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)
    Western blot - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)
    All lanes : Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197) at 1/500 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : POLB knockout HeLa cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa



    anes 1-3: Merged signal (red and green). Green - ab175197 observed at 38 kDa. Red - loading control, ab7291 observed at 50 kDa.

    ab175197 Anti-DNA Polymerase beta antibody [EPR12722(B)] was shown to specifically react with DNA Polymerase beta in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261748 (knockout cell lysate ab257096) was used. Wild-type and DNA Polymerase beta knockout samples were subjected to SDS-PAGE. ab175197 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti- Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti- Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)
    Western blot - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)
    All lanes : Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197) at 1/1000 dilution

    Lane 1 : HeLa cell lysates
    Lane 2 : Jurkat cell lysates
    Lane 3 : K562 cell lysates
    Lane 4 : A431 cell lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 38 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling DNA Polymerase beta with ab175197 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)

    Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling DNA Polymerase beta with ab175197 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)
    Immunocytochemistry/ Immunofluorescence - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)

    Immunofluorescence analysis of A431 cells labeling DNA Polymerase beta with ab175197 at a 1/50 dilution.

  • Immunoprecipitation - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)
    Immunoprecipitation - Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)

    Western blot analysis on immunoprecipitation pellet from K562 cell lysate labeling DNA Polymerase beta with ab175197 at 1/10 dilution.

  • Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)
    Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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