Anti-NF-kB p65 (phospho S276) antibody (ab194726)
Key features and details
- Rabbit polyclonal to NF-kB p65 (phospho S276)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-NF-kB p65 (phospho S276) antibody
See all NF-kB p65 primary antibodies -
Description
Rabbit polyclonal to NF-kB p65 (phospho S276) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide within Human NF-kB p65 (phospho S276). The exact sequence is proprietary.
Database link: Q04206 -
Positive control
- WB: HeLa and C6 cells treated with TNF-a lysates; IHC-P: Human breast, breast cancer and rat testis tissues; ICC/IF: U-2OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NF-kB p65 (phospho S276) antibody (ab194726)Anti-NF-kB p65 (phospho S276) antibody (ab194726) + HeLa cell extract at 25 µg
Secondary
HRP Goat Anti-Rabbit IgG.
Predicted band size: 60 kDaHeLa cells were treated by TNF-α (20ng/ml) for 30 minutes.
Blocking buffer: 3% BSA.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 (phospho S276) antibody (ab194726)Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling NF-kB p65 using ab194726 at 1/100 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 (phospho S276) antibody (ab194726)Immunocytochemistry/Immunofluorescent analysis of U-20S cells staining NF-kB p65 with ab194726 at 1/100 dilution. DAPI was used as a nuclear counterstain.
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Western blot - Anti-NF-kB p65 (phospho S276) antibody (ab194726)Anti-NF-kB p65 (phospho S276) antibody (ab194726) at 1/1000 dilution + C6 cells extracts at 25 µg
Secondary
HRP Goat Anti-Rabbit IgG
Developed using the ECL technique.
Predicted band size: 60 kDa
Exposure time: 90 secondsC6 cells were treated by TNF-α (20 ng/ml) at 37°C for 30 minutes after serumstarvation overnight.
Blocking buffer: 3% BSA.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 (phospho S276) antibody (ab194726)Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling NF-kB p65 using ab194726 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 (phospho S276) antibody (ab194726)Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling NF-kB p65 using ab194726 at 1/100 dilution.
