Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Lamin A + Lamin C antibody [EP4520-16] - BSA and Azide free (ab240053)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP4520-16] to Lamin A + Lamin C - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Lamin A + Lamin C antibody [EP4520-16] - BSA and Azide free
    See all Lamin A + Lamin C primary antibodies

  • Description

    Rabbit monoclonal [EP4520-16] to Lamin A + Lamin C - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Untreated HeLa, HeLa treated with staurosporine, HeLa treated with camptothecine, HAP1, HepG2, and Jurkat (ab7899) whole cell lysate. IHC-P: Human breast and human transitional cell carcinoma of bladder tissues.

  • General notes

    Ab240053 is the carrier-free version of ab133256. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240053 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Lamin A + Lamin C antibody [EP4520-16] - BSA and Azide free (ab240053)
    Western blot - Anti-Lamin A + Lamin C antibody [EP4520-16] - BSA and Azide free (ab240053)
    All lanes : Anti-Lamin A + Lamin C antibody [EP4520-16] (ab133256) at 1/5000 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : LMNA knockout HAP1 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 74 kDa
    Observed band size: 70-75 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab133256).

    Lanes 1 - 4: Merged signal (red and green). Green - ab133256 observed at 70-75 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab133256 was shown to react with LMNA in wild-type HAP1 cells in Western blot. Loss of signal was observed when LMNA knockout sample was used. HAP1 wild-type and LMNA knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab133256 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EP4520-16] - BSA and Azide free (ab240053)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EP4520-16] - BSA and Azide free (ab240053)

    Immunohistochemical analysis of paraffin-embedded Human breast tissue labelling Lamin A with ab133256 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133256).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EP4520-16] - BSA and Azide free (ab240053)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EP4520-16] - BSA and Azide free (ab240053)

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labelling Lamin A with ab133256 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133256).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Lamin A + Lamin C antibody [EP4520-16] - BSA and Azide free (ab240053)
    Anti-Lamin A + Lamin C antibody [EP4520-16] - BSA and Azide free (ab240053)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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