Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Lamin A + Lamin C antibody [EPR4519(2)] (ab169532)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4519(2)] to Lamin A + Lamin C
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-Lamin A + Lamin C antibody [EPR4519(2)]
See all Lamin A + Lamin C primary antibodies
Description

Rabbit monoclonal [EPR4519(2)] to Lamin A + Lamin C
Host species

Rabbit
Specificity

ab169532 recognizes full length Lamin A + C and cleaved large unit.

Tested Applications & Species

Application	Species
IHC-P	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human Lamin A + Lamin C.
Positive control
- WB: HAP1, HepG2, PC-12, THP-1, HeLa + Staurosporine and HeLa cell lysates. IHC-P: Human skin and Human uterus tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR4519(2)
Isotype

IgG
Research areas

Western blot - Anti-Lamin A + Lamin C antibody [EPR4519(2)] (ab169532)

All lanes : Anti-Lamin A + Lamin C antibody [EPR4519(2)] (ab169532) at 1/5000 dilution

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : LMNA knockout HAP1 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 74 kDa
Observed band size: 70-75 kDa
why is the actual band size different from the predicted?

Lanes 1 - 4: Merged signal (red and green). Green - ab169532 observed at 70-75 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab169532 was shown to react with LMNA in wild-type HAP1 cells in Western blot. Loss of signal was observed when LMNA knockout sample was used. HAP1 wild-type and LMNA knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween^®) before incubation with ab169532 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Lamin A + Lamin C antibody [EPR4519(2)] (ab169532)

All lanes : Anti-Lamin A + Lamin C antibody [EPR4519(2)] (ab169532) at 1/5000 dilution

Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse spleen tissue lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat heart tissue lysate
Lane 7 : Rat kidney tissue lysate
Lane 8 : Rat spleen tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution

Predicted band size: 74 kDa
Observed band size: 74 kDa

Exposure time: 30 seconds

Blocking and Diluting buffer 5% NFDM /TBST

65-74 kDa: full length;
45-50 kDa: cleavage forms
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EPR4519(2)] (ab169532)

ab169532 showing +ve staining in Human normal colon tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EPR4519(2)] (ab169532)

ab169532 showing +ve staining in Human normal brain tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EPR4519(2)] (ab169532)

ab169532 showing +ve staining in Human normal kidney tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EPR4519(2)] (ab169532)

ab169532 showing +ve staining in Human normal tonsil tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EPR4519(2)] (ab169532)

Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Lamin A + C with ab169532 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin A + Lamin C antibody [EPR4519(2)] (ab169532)

Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling Lamin A + C with ab169532 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-Lamin A + Lamin C antibody [EPR4519(2)] (ab169532)

All lanes : Anti-Lamin A + Lamin C antibody [EPR4519(2)] (ab169532) at 1/10000 dilution

Lane 1 : PC-12 cell lysate
Lane 2 : THP-1 cell lysate
Lane 3 : HeLa + Staurosporine cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 74 kDa
Anti-Lamin A + Lamin C antibody [EPR4519(2)] (ab169532)

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