Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Iba1 antibody [EPR6136(2)] (ab178680)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6136(2)] to Iba1
  • Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Iba1 antibody [EPR6136(2)]
    See all Iba1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6136(2)] to Iba1

  • Host species

    Rabbit

  • Specificity

    In our hands, ab178680 detects a band at the correct molecular weight, 17 kDa, in human tissue and cell lines in WB. However it did not detect Iba1 in rat or mouse samples. Abcam recommends blocking in 3% milk for this product. Blocking in BSA gives high background.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Iba1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P55008

  • Positive control

    • IHC-P: Human glioma, hippocampal and kidney tissues; ICC/IF: K562 cells; Flow Cyt: K562 cells; WB: Human fetal spleen lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
    Western blot - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
    Anti-Iba1 antibody [EPR6136(2)] (ab178680) at 1/1000 dilution + Human fetal spleen lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 16 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?



    Exposure time: 180 seconds.

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)

    IHC image of Iba1 staining in human normal hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab178680, 1/500 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)

    Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling Iba1 with ab178680 at 1/250 dilution.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR6136(2)] (ab178680)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Iba1 with ab178680 at 1/250 dilution.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
    Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [EPR6136(2)] (ab178680)

    Immunofluorescent analysis of K562 cells labeling Iba1 with ab178680 (green). DAPI nuclear staining (blue).

  • Flow Cytometry - Anti-Iba1 antibody [EPR6136(2)] (ab178680)
    Flow Cytometry - Anti-Iba1 antibody [EPR6136(2)] (ab178680)

    Flow cytometric analysis of permeabilized K562 cells labeling Iba1 with ab178680 at 1/100 dilution (red) or a rabbit IgG (negative) (green).

  • Anti-Iba1 antibody [EPR6136(2)] (ab178680)
    Anti-Iba1 antibody [EPR6136(2)] (ab178680)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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