Anti-Iba1 antibody (ab5076)
Key features and details
- Goat polyclonal to Iba1
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Iba1 antibody
See all Iba1 primary antibodies -
Description
Goat polyclonal to Iba1 -
Host species
Goat -
Specificity
ab5076 is expected to recognize the isoforms represented by NP_001614 and NP_116573 but not NP_004838.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P RatHumanWB RatHuman
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Immunogen
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Positive control
- WB: Human lymph node and rat brain tissue lysate. IHC-P: Human placenta tissue.
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General notes
The Iba1 antibody (ab5076) is commonly used as a marker of microglia activation in staining and immunohistochemistry, given that ionized calcium binding adaptor molecule 1 (Iba1) is a microglia/macrophage-specific calcium-binding protein with actin-bundling activity that participates in membrane ruffling and phagocytosis in activated microglia.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Iba1 antibody (ab5076)All lanes : Anti-Iba1 antibody (ab5076) at 1 µg/ml
Lane 1 : Human lymph node tissue lysate in RIPA buffer
Lane 2 : Rat brain tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 17 kDaDetected by chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab5076)Immunohistochemistry analysis of paraffin-embedded human placenta tissue labeling Iba1 with ab5076 at 6 µg/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab5076) Image from Iris Margalit Trutzer et al, ACTA neuropathologica communications, 7, 40, Fig. 7; doi: 10.1186/s40478-019-0684-8Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lateral prefrontal cortex tissue labeling iba1 labeled microglia (labeled with black arrows) within the cortex using ab5076 at 1/1000 dilution. (Cropped image).
Sections were rinsed in 0.01 m PBS, pH 7.4, followed by 10% serum matching the species of the secondary antibody, 5% bovine serum albumin, and 0.1% Triton X-100 in 0.01 m PBS blocking solution for 1 h and incubated for 2 days at 4 °C in primary antibody. The sections were rinsed in PBS and incubated overnight at 4 °C with a horse anti-Goat IgG (Biotinylated) secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody (ab5076) This image is courtesy of an abreview submitted by Carl Hobbs (King's College London, United Kingdom)IHC-P image of Iba1 staining on rat brain sections using ab5076 (1:1000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab5076 was diluted 1:1000 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was Rabbit polyclonal to anti sheep/goat IgG conjugated to biotin (1:200)
