Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FANCI antibody (ab103077)

Key features and details

  • Rabbit polyclonal to FANCI
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-FANCI antibody
    See all FANCI primary antibodies

  • Description

    Rabbit polyclonal to FANCI

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human FANCI aa 950-1050 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab124620)

  • Positive control

    • WB: Raji, HeLa, HEK293, U20S and MCF7 cell lysates.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab103077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Mouse
Horse
Macaque monkey
Gorilla
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 151 kDa (predicted molecular weight: 149 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 151 kDa (predicted molecular weight: 149 kDa).

Target

  • Function

    Required for maintenance of chromosomal stability. Involved in the repair of DNA double-strand breaks by homologous recombination and in the repair of DNA cross-links. Participates in S phase and G2 phase checkpoint activation upon DNA damage. Promotes FANCD2 ubiquitination and recruitment to DNA repair sites.

  • Involvement in disease

    Defects in FANCI are a cause of Fanconi anemia complementation group I (FANCI) [MIM:609053]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.

  • Domain

    The C-terminal 30 residues are probably required for function in DNA repair.

  • Post-translational
    modifications

    Monoubiquitinated on Lys-523 during S phase and upon genotoxic stress. Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex. Ubiquitination is required for binding to chromatin, DNA repair, and normal cell cycle progression.
    Phosphorylated in response to DNA damage by ATM and/or ATR.

  • Cellular localization

    Nucleus. Concentrates in nuclear foci upon genotoxic stress.
  • Information by UniProt

  • Database links

    • Alternative names

      • FANCI antibody
      • FANCI gene antibody
      • FANCI_HUMAN antibody
      • Fanconi anemia group I protein antibody
      • Fanconi anemia, complementation group I antibody
      • FLJ10719 antibody
      • FLJ14658 antibody
      • KIAA1794 antibody
      • Protein FACI antibody
      • Protein FANCI antibody
      see all

    Images

    • Western blot - Anti-FANCI antibody (ab103077)
      Western blot - Anti-FANCI antibody (ab103077)
      All lanes : Anti-FANCI antibody (ab103077) at 1/500 dilution

      Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : FANCI knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 4 : Raji (Human Burkitts lymphoma cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 149 kDa
      Observed band size: 151 kDa why is the actual band size different from the predicted?



      Lanes 1-4: Merged signal (red and green). Green - ab103077 observed at 151 kDa. Red - loading control ab8245 observed at 36 kDa.

       ab103077 Anti-FANCI antibody was shown to specifically react with FANCI in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266278 (knockout cell lysate ab257434) was used. Wild-type and FANCI knockout samples were subjected to SDS-PAGE. ab103077 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-FANCI antibody (ab103077)
      Western blot - Anti-FANCI antibody (ab103077)
      All lanes : Anti-FANCI antibody (ab103077) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
      Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 149 kDa
      Observed band size: 151 kDa why is the actual band size different from the predicted?
      Additional bands at: 49 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes

    References (0)

    Publishing research using ab103077? Please let us know so that we can cite the reference in this datasheet.

    ab103077 has not yet been referenced specifically in any publications.

    Images

    • Western blot - Anti-FANCI antibody (ab103077)
      Western blot - Anti-FANCI antibody (ab103077)
      All lanes : Anti-FANCI antibody (ab103077) at 1/500 dilution

      Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : FANCI knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 4 : Raji (Human Burkitts lymphoma cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 149 kDa
      Observed band size: 151 kDa
      why is the actual band size different from the predicted?



      Lanes 1-4: Merged signal (red and green). Green - ab103077 observed at 151 kDa. Red - loading control ab8245 observed at 36 kDa.

       ab103077 Anti-FANCI antibody was shown to specifically react with FANCI in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266278 (knockout cell lysate ab257434) was used. Wild-type and FANCI knockout samples were subjected to SDS-PAGE. ab103077 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-FANCI antibody (ab103077)
      Western blot - Anti-FANCI antibody (ab103077)
      All lanes : Anti-FANCI antibody (ab103077) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
      Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 149 kDa
      Observed band size: 151 kDa why is the actual band size different from the predicted?
      Additional bands at: 49 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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