Anti-CSB antibody [553C5a] (ab66598)
![Anti-CSB antibody [553C5a] (ab66598)](https://www.abcam.com/ps/products/66/ab66598/Images/ab66598-204504-anti-csb-antibody-553c5a-flow-cytometry.jpg "Anti-CSB antibody [553C5a] (ab66598)")
Key features and details
- Mouse monoclonal [553C5a] to CSB
- Suitable for: Flow Cyt, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2b
Overview
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Product name
Anti-CSB antibody [553C5a]
See all CSB primary antibodies -
Description
Mouse monoclonal [553C5a] to CSB -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment corresponding to an internal region carrying 50-200 aa of Human CSB
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.0225% Potassium chloride, 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 0.812% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22 µm membrane. -
Clonality
Monoclonal -
Clone number
553C5a -
Isotype
IgG2b -
Research areas
Images
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Flow Cytometry - Anti-CSB antibody [553C5a] (ab66598)Overlay histogram showing HeLa cells stained with ab66598 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab66598, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Western blot - Anti-CSB antibody [553C5a] (ab66598)](https://www.abcam.com/ps/products/66/ab66598/Images/ab66598-80485-anti-csb-antibody-553c5a-western-blot.jpg)
Western blot - Anti-CSB antibody [553C5a] (ab66598)Anti-CSB antibody [553C5a] (ab66598) + immunising recombinant protein
Predicted band size: 168 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Primary antibody dilution 1:100, Recombinant protein amount 10ng/lane, Secondary antibody dilution 1:3000, Secondary antibody Sheep anti mouse IgG (0.63mg/ml)
