Anti-TGF beta 2 antibody (ab113670)
Key features and details
- Rabbit polyclonal to TGF beta 2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TGF beta 2 antibody
See all TGF beta 2 primary antibodies -
Description
Rabbit polyclonal to TGF beta 2 -
Host species
Rabbit -
Specificity
This antibody is raised against the latency associated peptide (LAP) region of TGFB2, so it will not recognize mature TGFB2. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Macaque monkey, Gorilla
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MCF7 whole cell lysate and human breast, human adipose, rat kidney and mouse kidney tissue lysates. ICC/IF: HepG2 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab113670 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa). ICC/IF Use a concentration of 5 µg/ml. Target
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Function
TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth. -
Involvement in disease
A chromosomal aberration involving TGFB2 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with HDAC9.
Loeys-Dietz syndrome 4
Defects in TGFB2 may be a cause of non-syndromic aortic disease (NSAD). NSAD is a frequently asymptomatic but potentially lethal disease characterized by thoracic aortic aneurysms and dissections without additional syndromic features. -
Sequence similarities
Belongs to the TGF-beta family. -
Post-translational
modificationsThe precursor is cleaved into mature TGF-beta-2 and LAP, which remains non-covalently linked to mature TGF-beta-2 rendering it inactive. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 534069 Cow
- Entrez Gene: 7040 Human
- Entrez Gene: 7042 Human
- Entrez Gene: 7043 Human
- Entrez Gene: 21803 Mouse
- Entrez Gene: 21808 Mouse
- Entrez Gene: 397084 Pig
- Entrez Gene: 100009371 Rabbit
see all -
Alternative names
- BSC-1 cell growth inhibitor antibody
- BSC1 cell growth inhibitor antibody
- Cetermin antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-TGF beta 2 antibody (ab113670)
ICC/IF image of ab113670 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab113670, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-TGF beta 2 antibody (ab113670)All lanes : Anti-TGF beta 2 antibody (ab113670) at 1 µg/ml
Lane 1 : Human breast tissue lysate - total protein (ab30090)
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : Kidney (Mouse) Tissue Lysate
Lane 4 : Kidney (Rat) Tissue Lysate
Lane 5 : Human adipose normal tissue lysate - total protein (ab28980)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 68 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 150 seconds
Protocols
Datasheets and documents
References (6)
ab113670 has been referenced in 6 publications.
- Zhu HP et al. Regulatory mechanism of NOV/CCN3 in the inflammation and apoptosis of lung epithelial alveolar cells upon lipopolysaccharide stimulation. Mol Med Rep 21:1872-1880 (2020). PubMed: 31545412
- MacFarlane EG et al. Lineage-specific events underlie aortic root aneurysm pathogenesis in Loeys-Dietz syndrome. J Clin Invest 129:659-675 (2019). PubMed: 30614814
- Liu J et al. The role of focal adhesion kinase in transforming growth factor-ß2 induced migration of human lens epithelial cells. Int J Mol Med 42:3591-3601 (2018). PubMed: 30280182
- Reithmeier A et al. Tartrate-resistant acid phosphatase (TRAP/ACP5) promotes metastasis-related properties via TGFß2/TßR and CD44 in MDA-MB-231 breast cancer cells. BMC Cancer 17:650 (2017). PubMed: 28915803
- Zhang Q et al. The relationship between anti-vascular endothelial growth factor and fibrosis in proliferative retinopathy: clinical and laboratory evidence. Br J Ophthalmol 100:1443-50 (2016). PubMed: 27531356
- Florea V et al. c-Myc Is Essential to Prevent Endothelial Pro-Inflammatory Senescent Phenotype. PLoS One 8:e73146 (2013). PubMed: 24039874
Images
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Immunocytochemistry/ Immunofluorescence - Anti-TGF beta 2 antibody (ab113670)
ICC/IF image of ab113670 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab113670, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-TGF beta 2 antibody (ab113670)All lanes : Anti-TGF beta 2 antibody (ab113670) at 1 µg/ml
Lane 1 : Human breast tissue lysate - total protein (ab30090)
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : Kidney (Mouse) Tissue Lysate
Lane 4 : Kidney (Rat) Tissue Lysate
Lane 5 : Human adipose normal tissue lysate - total protein (ab28980)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 68 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 150 seconds
