Anti-Haptoglobulin beta antibody (ab126842)
Key features and details
- Goat polyclonal to Haptoglobulin beta
- Suitable for: WB
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-Haptoglobulin beta antibody
See all Haptoglobulin beta primary antibodies -
Description
Goat polyclonal to Haptoglobulin beta -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species WB HumanPig
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Immunogen
Synthetic peptide:
C-STVPEKKTPKSP
(with a Cysteine residue linker), corresponding to internal sequence amino acids 316-327 of Human Haptoglobulin beta (NP_005134.1; NP_001119574.1). -
Positive control
- Human Liver lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Haptoglobulin beta antibody (ab126842)Anti-Haptoglobulin beta antibody (ab126842) at 0.3 µg/ml + pig liver tissue lysate
Predicted band size: 45 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
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Western blot - Anti-Haptoglobulin beta antibody (ab126842)Anti-Haptoglobulin beta antibody (ab126842) at 0.1 µg/ml + Human Liver lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 45 kDa
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Western blot - Anti-Haptoglobulin beta antibody (ab126842) This image is courtesy of an anonymous AbreviewLanes 2-3 : Anti-Haptoglobulin beta antibody (ab126842) at 1/20 dilution
Lane 1 : Marker
Lanes 2-3 : Pig endometrial tissue lysate - whole at 30 µg
Secondary
Lanes 2-3 : Alkaline phosphatase rabbit anti-goat IgG polyclonal at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Additional bands at: 27 kDa (possible non-specific binding), 52 kDa (possible non-specific binding)
Exposure time: 24 minutes52kDa band is from the secondary antibody.
