Mouse Granzyme A ELISA Kit (ab253213)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 11 pg/ml
- Range: 39.1 pg/ml - 2500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Granzyme A ELISA Kit
See all Granzyme A kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 1.9% Inter-assay Sample n Mean SD CV% Extract 3 16% -
Sample type
Cell culture supernatant, Serum, Tissue Extracts, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
11 pg/ml -
Range
39.1 pg/ml - 2500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 105 98% - 109% Serum 80 79% - 81% Tissue Extracts 82 76% - 89% EDTA Plasma 109 105% - 114% Cit plasma 97 96% - 99% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Granzyme A ELISA Kit (ab253213) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Granzyme A protein in cell culture supernatant, cit plasma, edta plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Granzyme A with 11 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Granzyme A, also known as T cell-specific serine protease 1, is a trypsin-like serine protease that forms a disulfide-linked homodimer and is secreted by cytotoxic T lymphocytes and natural killer cells. While Granzyme A is the most abundant serine protease in killer cell granules, it is considered less cytotoxic than Granzyme B, but can activate the caspase-independent cell death with morphological features of apoptosis. Granzyme A targets several nuclear proteins for degradation including, Pro-IL-1beta, NDUFS3, SET, AP1, Histone H1, HMGB2, PARP-1, Ku70, and lamins, thus limiting DNA repair, oxidative repair activity, and disrupting the election transport of mitochondria. Granzyme A is also considered to have extracellular functions including promoting inflammation and degradation of extracellular matrix.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Granzyme A Capture Antibody 1 x 600µl 10X Mouse Granzyme A Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse Granzyme A Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Involved in apoptosis. -
Sequence similarities
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
Cellular localization
Secreted. Cytoplasmic granule. - Information by UniProt
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Alternative names
- CTL tryptase
- CTLA3
- Cytolytic t cell and natural killer cell specific trypsin like serine protease
see all -
Database links
- Entrez Gene: 14938 Mouse
- SwissProt: P11032 Mouse
- Unigene: 15510 Mouse
Images
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Other - Mouse Granzyme A ELISA Kit (ab253213)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Granzyme A standard curve in Sample Diluent NS.The Granzyme A standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse Granzyme A standard curve in Sample Diluent 50BS.The Granzyme A standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse Granzyme A standard curve in 1X Cell Extraction Buffer PTR.The Granzyme A standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Granzyme A in mousesplenocytes culture supernatant samples.The concentrations of Granzyme A were measured in duplicates, interpolated from the Granzyme A standard curves and corrected for sample dilution. Undiluted samples are as follows: spleen supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granzyme A concentration was determined to be 615 pg/mL in neat splenocyte culture supernatant.
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Interpolated concentrations of native Granzyme A in mouse spleen extract based on a 1,000 µg/mL extract load.The concentrations of Granzyme A were measured in duplicate and interpolated from the Granzyme A standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granzyme A concentration was determined to be 1,424 pg/mL in spleen extract.
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Sandwich ELISA - Mouse Granzyme A ELISA Kit (ab253213)To learn more about the advantages of recombinant antibodies see here.
