Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16750] to 14-3-3 eta/YWHAH - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free
    See all 14-3-3 eta/YWHAH primary antibodies

  • Description

    Rabbit monoclonal [EPR16750] to 14-3-3 eta/YWHAH - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251443 is the carrier-free version of ab206292 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251443 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as 14-3-3 eta

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)
    Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)
    All lanes : Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292) at 1/5000 dilution

    Lane 1 : Human fetal brain tissue lysate
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa



    This data was developed using ab206292, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 15 seconds; Lanes 2-3: 3 minutes.

  • Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)
    Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)
    All lanes : Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse spleen tissue lysate
    Lane 3 : Rat brain tissue lysate
    Lane 4 : Rat spleen tissue lysate
    Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 7 : Mouse heart tissue lysate
    Lane 8 : Mouse kidney tissue lysate
    Lane 9 : Rat heart tissue lysate
    Lane 10 : Rat kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa



    This data was developed using ab206292, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1-6: 30 seconds; Lanes 7 -10: 3 minutes.

  • Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)
    Western blot - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)
    All lanes : Anti-14-3-3 eta/YWHAH antibody [EPR16750] (ab206292) at 1/2000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cytosolic fraction
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear fraction

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa


    Exposure time: 3 minutes


    This data was developed using ab206292, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)
    Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)
    This data was developed using ab206292, the same antibody clone in a different buffer formulation.14-3-3 eta/YWHAH was immunoprecipitated from 1mg of Human brain whole cell lysate with ab206292 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab206292 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: Human brain whole cell lysate 10ug (Input). Lane 2: ab206292 IP in Human brain whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206292 in Human brain whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 30 seconds.
  • Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)
    Immunoprecipitation - Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)
    This data was developed using ab206292, the same antibody clone in a different buffer formulation.Negative control: 14-3-3 eta/YWHAH was immunoprecipitated from 1mg of Human brain whole cell lysate with ab206292 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab137106 (which is specific to 14-3-3 gamma) at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: Human brain whole cell lysate 10ug (Input). Lane 2: ab206292 IP in Human brain whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206292 in Human brain whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 30 seconds.
  • Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)
    Anti-14-3-3 eta/YWHAH antibody [EPR16750] - BSA and Azide free (ab251443)

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