Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-FUBP1/FBP antibody [EPR19208] - BSA and Azide free
See all FUBP1/FBP primary antibodies
Description

Rabbit monoclonal [EPR19208] to FUBP1/FBP - BSA and Azide free
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Mouse
IP	Human
WB	Rat

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab251543 is the carrier-free version of ab213525. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251543 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Clonality

Monoclonal
Clone number

EPR19208
Isotype

IgG
Research areas

Western blot - Anti-FUBP1/FBP antibody [EPR19208] - BSA and Azide free (ab251543)

All lanes : Anti-FUBP1/FBP antibody [EPR19208] (ab213525) at 1/5000 dilution

Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 68 kDa
Observed band size: 74 kDa
why is the actual band size different from the predicted?

Exposure time: 1 second

This data was developed using ab213525, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

The expression pattern observed is consistent with the literature (PMID: 24963357).
Western blot - Anti-FUBP1/FBP antibody [EPR19208] - BSA and Azide free (ab251543)

Lanes 1-2 : Anti-FUBP1/FBP antibody [EPR19208] (ab213525) at 1/1000 dilution
Lanes 3-6 : Anti-FUBP1/FBP antibody [EPR19208] (ab213525) at 1/5000 dilution

Lane 1 : Rat brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 6 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 68 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?

This data was developed using ab213525, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times: Lanes 1-3, 5, and 6: 1 second; Lane 4: 3 seconds.

The expression pattern observed is consistent with the literature (PMID: 24963357).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR19208] - BSA and Azide free (ab251543)

This data was developed using ab213525, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human oligodendroglioma tissue labeling FUBP1/FBP with ab213525 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on the vascular endothelium of human oligodendroglioma is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR19208] - BSA and Azide free (ab251543)

This data was developed using ab213525, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling FUBP1/FBP with ab213525 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on the neurons of human cerebral cortex is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR19208] - BSA and Azide free (ab251543)

This data was developed using ab213525, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling FUBP1/FBP with ab213525 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on the neurons of mouse cerebral cortex is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR19208] - BSA and Azide free (ab251543)

This data was developed using ab213525, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling FUBP1/FBP with ab213525 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on the neurons of rat stomach is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry - Anti-FUBP1/FBP antibody [EPR19208] - BSA and Azide free (ab251543)

This data was developed using ab213525, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling FUBP1/FBP with ab213525 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Immunocytochemistry - Anti-FUBP1/FBP antibody [EPR19208] - BSA and Azide free (ab251543)

This data was developed using ab213525, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling FUBP1/FBP with ab213525 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on NIH/3T3 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Flow Cytometry - Anti-FUBP1/FBP antibody [EPR19208] - BSA and Azide free (ab251543)

This data was developed using ab213525, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling FUBP1/FBP with ab213525 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-FUBP1/FBP antibody [EPR19208] - BSA and Azide free (ab251543)

This data was developed using ab213525, the same antibody clone in a different buffer formulation.FUBP1/FBP was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab213525 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab213525 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Lane 1: HeLa whole cell lysate 10µg (Input). Lane 2: ab213525 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213525 in HeLa whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 1 second. The expression pattern observed is consistent with the literature (PMID: 24963357).
Anti-FUBP1/FBP antibody [EPR19208] - BSA and Azide free (ab251543)