Nb2 nuclear extract lysate (ab14868)
Overview
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Product name
Nb2 nuclear extract lysate
See all Nb2 lysates -
General notes
The protein content has been determined by a Bradford-based assay.
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.90
Constituents: 0.75% Potassium chloride, 0.0087% PMSF, 0.0095% Magnesium chloride, 0.0077% DTT, 0.476% HEPES, 20% Glycerol -
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Lysate notes
Nb2 nuclear extract lysate was prepared from cell cultures of the rat lymphoma Nb2 nuclear cell line. The Nb2 cell line was derived from the lymphoma transplant in the thymus of a noble (Nb) strain male rat following prolonged estrogen treatment
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Research areas
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Background
The Nb-2 rat lymphoma line was derived from a transplant of a lymphoma that developed in the thymus/lymph node of a male noble (Nb) strain rat following prolonged oestrogen treatment. The cells are of the pre-T cell origin and their proliferation is dependent on mammalian lactogens, such as prolactin. Cells have a modified prolactin receptor and are used for in vitro bioassay of lactogenic hormones, studies of the mechanisms of lactogen induced mitogenesis, studies into the action of vinca alkaloids and studies of tumour progression.