Recombinant Human Sonic Hedgehog protein (ab174054)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Reconstitute for long term storage.
pH: 7.40
Constituents: 5% Trehalose, 95% PBS -
ReconstitutionIt is recommended to reconstitute the lyophilized product in 50 µl sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
Images
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SDS-PAGE - Recombinant Human Sonic Hedgehog protein (ab174054)
SDS-PAGE of reduced ab174054 stained overnight with Coomassie Blue. The protein migrates as 23-25 kDa due to glycosylation.
