Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant human Thrombopoietin protein (ab9719)

Key features and details

  • Expression system: Escherichia coli
  • Endotoxin level:
  • Active: Yes
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human Thrombopoietin protein
    See all Thrombopoietin proteins and peptides

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      Recombinant human TPO is a fully biologically active 174 amino acid polypeptide (18.6 kDa), which contains the erythropoietin-like domain of the full length TPO protein. SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL

    • Associated products

      Specifications

      Our Abpromise guarantee covers the use of ab9719 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      • Applications

        Functional Studies

        SDS-PAGE

      • Form

        Lyophilized

      • Additional notes

        The ED50, determined by the dose-dependent stimulation of the proliferation of the human MO7e cells, is 1 x 106 units/mg
      • Concentration information loading...

      Preparation and Storage

      • Stability and Storage

        Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

        This product is an active protein and may elicit a biological response in vivo, handle with caution.

      • Reconstitution
        For lot specific reconstitution information please contact our Scientific Support Team.

      General Info

      • Alternative names

        • C mpl ligand
        • C-mpl ligand
        • Megakaryocyte colony stimulating factor
        • Megakaryocyte colony-stimulating factor
        • Megakaryocyte growth and development factor
        • Megakaryocyte stimulating factor
        • MGC163194
        • MGDF
        • MKCSF
        • ML
        • MPL ligand
        • MPLLG
        • Myeloproliferative leukemia virus oncogene ligand
        • Prepro thrombopoietin
        • THCYT1
        • THPO
        • Thrombopoietin
        • Thrombopoietin nirs variant 1
        • TPO
        • TPO_HUMAN
        see all

      • Function

        Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.

      • Involvement in disease

        Defects in THPO are a cause of essential thrombocythemia (ET) [MIM:187950]. ET is inherited as an autosomal dominant trait which is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications.

      • Sequence similarities

        Belongs to the EPO/TPO family.

      • Domain

        Two-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal.

      • Cellular localization

        Secreted.
      • Information by UniProt

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab9719? Please let us know so that we can cite the reference in this datasheet.

    ab9719 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • Alternative names

      • C mpl ligand
      • C-mpl ligand
      • Megakaryocyte colony stimulating factor
      • Megakaryocyte colony-stimulating factor
      • Megakaryocyte growth and development factor
      • Megakaryocyte stimulating factor
      • MGC163194
      • MGDF
      • MKCSF
      • ML
      • MPL ligand
      • MPLLG
      • Myeloproliferative leukemia virus oncogene ligand
      • Prepro thrombopoietin
      • THCYT1
      • THPO
      • Thrombopoietin
      • Thrombopoietin nirs variant 1
      • TPO
      • TPO_HUMAN
      see all

    • Function

      Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.

    • Involvement in disease

      Defects in THPO are a cause of essential thrombocythemia (ET) [MIM:187950]. ET is inherited as an autosomal dominant trait which is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications.

    • Sequence similarities

      Belongs to the EPO/TPO family.

    • Domain

      Two-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal.

    • Cellular localization

      Secreted.
    • Information by UniProt

    Images

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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