Recombinant mouse Thrombopoietin protein (Animal Free) (ab217465)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: HPLC, Functional Studies, SDS-PAGE
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Product name
Recombinant mouse Thrombopoietin protein (Animal Free)
See all Thrombopoietin proteins and peptides -
Biological activity
The ED50 as determined by the dose-dependent stimulation of the proliferation of human MO7e cells was found to be ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.
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Purity
> 98 % SDS-PAGE.
> 98 % by HPLC. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
Yes -
Nature
Recombinant -
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Species
Mouse -
Sequence
SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKF -
Predicted molecular weight
19 kDa -
Amino acids
22 to 195
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Preparation and Storage
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Alternative names
- C mpl ligand
- C-mpl ligand
- Megakaryocyte colony stimulating factor
see all -
Function
Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. -
Involvement in disease
Defects in THPO are a cause of essential thrombocythemia (ET) [MIM:187950]. ET is inherited as an autosomal dominant trait which is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. -
Sequence similarities
Belongs to the EPO/TPO family. -
Domain
Two-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal. -
Cellular localization
Secreted. - Information by UniProt
