Anti-SERPINB1/PI2 antibody [EPR13299] - Membrane Vesicle Marker (ab190357)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13299] to SERPINB1/PI2 - Membrane Vesicle Marker
- Suitable for: Flow Cyt, WB
- Reacts with: Human
Overview
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Product name
Anti-SERPINB1/PI2 antibody [EPR13299] - Membrane Vesicle Marker
See all SERPINB1/PI2 primary antibodies -
Description
Rabbit monoclonal [EPR13299] to SERPINB1/PI2 - Membrane Vesicle Marker -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal kidney, Human fetal lung, Human fetal liver and Human stomach lysates; HepG2, A549 and MCF7 cell lysates; U937 cells.
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General notes
This product was previously labelled as SERPINB1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13299 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-SERPINB1/PI2 antibody [EPR13299] - Membrane Vesicle Marker (ab190357) at 1/5000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
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All lanes : Anti-SERPINB1/PI2 antibody [EPR13299] - Membrane Vesicle Marker (ab190357) at 1/5000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal lung lysate
Lane 3 : Human fetal liver lysate
Lane 4 : Human stomach lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
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Flow Cytometrical analysis of U937 cells labeling SERPINB1/PI2 with ab190357 at 1/210 (red) compared to a Rabbit monoclonal IgG as an isotope control (green). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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