Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Angiotensinogen antibody [EPR20599] (ab213705)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20599] to Angiotensinogen
Suitable for: WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-Angiotensinogen antibody [EPR20599]
See all Angiotensinogen primary antibodies
Description

Rabbit monoclonal [EPR20599] to Angiotensinogen
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Mouse

WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Hepa1-6, mIMCD3, Neuro-2a, HepG2, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse and rat liver, brain and heart lysates; Human fetal heart and fetal kidney lysates. IP: Mouse liver lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Mouse
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20599
Isotype

IgG
Research areas

Western blot - Anti-Angiotensinogen antibody [EPR20599] (ab213705)

All lanes : Anti-Angiotensinogen antibody [EPR20599] (ab213705) at 1/1000 dilution

Lane 1 : Hepa1-6 (Mouse hepatoma epithelial cell line) whole cell lysate
Lane 2 : mIMCD3 (Mouse inner medullary collecting duct epithelial cell line) whole cell lysate
Lane 3 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 5 : Mouse liver lysate
Lane 6 : Rat liver lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 51 kDa
Observed band size: 51 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1-4: 1 minute; Lane 5: 5 seconds; Lane 6: 3 seconds.

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 22518004).
Western blot - Anti-Angiotensinogen antibody [EPR20599] (ab213705)

All lanes : Anti-Angiotensinogen antibody [EPR20599] (ab213705) at 1/1000 dilution

Lane 1 : Rat brain lysate
Lane 2 : Rat heart lysate
Lane 3 : Mouse brain lysate
Lane 4 : Mouse heart lysate
Lane 5 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 7 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 51 kDa
Observed band size: 51 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1-4: 1 second; Lane 5-7: 15 seconds.

The expression profile observed is consistent with what has been described in the literature for mouse heart (PMID: 25085967) or brain (PMID:21628469).
Western blot - Anti-Angiotensinogen antibody [EPR20599] (ab213705)

All lanes : Anti-Angiotensinogen antibody [EPR20599] (ab213705) at 1/1000 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

Predicted band size: 51 kDa
Observed band size: 51 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-Angiotensinogen antibody [EPR20599] (ab213705)

Angiotensinogen was immunoprecipitated from 0.35 mg of mouse liver lysate with ab213705 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab213705 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

Lane 1: Mouse liver lysate 10 µg (Input).

Lane 2: ab213705 IP in mouse liver lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213705 in mouse liver lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Anti-Angiotensinogen antibody [EPR20599] (ab213705)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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