Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Angiotensinogen antibody [EPR2931] (ab108334)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2931] to Angiotensinogen
Suitable for: WB, IP, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-Angiotensinogen antibody [EPR2931]
See all Angiotensinogen primary antibodies
Description

Rabbit monoclonal [EPR2931] to Angiotensinogen
Host species

Rabbit
Specificity

ab108334 is raised against the Human Angiotensinogen protein. This has little homology with mouse or rat Angiotensinogen and in our hands we do not get good results when testing in these species (in western blot the band is weak or absent and at a lower molecular weight than in human samples) therefore we cannot guarantee this product for use in these species. When using this antibody for western blot we recommend blocking with 5% BSA for best results. In our hands, blocking with milk does not give optimal results in WB and some lower molecular weight binding can be observed.
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Rat

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human fetal artery, fetal heart, fetal liver, adult kidney, HepG2, 293T, and plasma lysates; HepG2 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human
WB	Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR2931
Isotype

IgG
Research areas

Western blot - Anti-Angiotensinogen antibody [EPR2931] (ab108334)

All lanes : Anti-Angiotensinogen antibody [EPR2931] (ab108334) at 1/1000 dilution

Lane 1 : Mouse kidney whole cell lysate
Lane 2 : Rat kidney whole cell lysate
Lane 3 : Human kidney whole cell lysate
Lane 4 : Mouse liver whole cell lysate
Lane 5 : Rat liver whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit IgG (H+L) peroxidase conjugated. at 1/2000 dilution

Predicted band size: 53 kDa

Exposure time: 1 minute

Ab108334 targeting angiotensinogen in various mouse, rat and human tissues (see lane descriptions).

Blocking buffer for the first lanes 1-5: 5% NFDM/TBST; the second lanes 1-5: BSA/TBST.
Western blot - Anti-Angiotensinogen antibody [EPR2931] (ab108334)

All lanes : Anti-Angiotensinogen antibody [EPR2931] (ab108334) at 1/1000 dilution

All lanes : Human fetal liver whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit IgG (H+L) peroxidase conjugated. at 1/2000 dilution

Predicted band size: 53 kDa

Exposure time: 3 minutes

Lane 1 blocking buffer: NFDM/TBST

Lane 2 blocking buffer: BSA/TBST
Western blot - Anti-Angiotensinogen antibody [EPR2931] (ab108334)

All lanes : Anti-Angiotensinogen antibody [EPR2931] (ab108334) at 1/1000 dilution

Lane 1 : Human fetal artery lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal liver lysate
Lane 4 : HepG2 lysate
Lane 5 : 293T lysate
Lane 6 : Human plasma lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 53 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Angiotensinogen antibody [EPR2931] (ab108334)

ab108334, at 1/100 dilution, staining Angiotensinogen in HepG2 cells by Immunofluorescence.
Anti-Angiotensinogen antibody [EPR2931] (ab108334)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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